M3024
Terreno essenziale minimo di Eagle
with Earle′s salts and non-essential amino acids, without L-glutamine, phenol red and sodium bicarbonate, Modified, powder, suitable for cell culture
Sinonimo/i:
MEM
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About This Item
Codice UNSPSC:
12352207
NACRES:
NA.75
Livello qualitativo
Forma fisica
powder
tecniche
cell culture | mammalian: suitable
Componenti
Earle’s salts (5% CO2): yes
L-glutamine: no
HEPES: no
NaHCO3: no
sodium pyruvate: no
phenol red: no
Condizioni di spedizione
ambient
Temperatura di conservazione
2-8°C
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Descrizione generale
Minimum Essential Medium Eagle contains 19 amino acids. The essential amino acids and the non-essential amino acids: L-ala, L-asn, L-asp, L-glu, L-gly, L-pro, and L-ser. This medium is preferred for the growth of adherent cells in the presence of reduced serum or as a base for the development of a serum-free medium. It is suitable for a wide variety of cells grown in monolayers.
Applicazioni
Minimum Essential Medium Eagle has been used:
- to culture endometrial adenocarcinoma Ishikawa cells for bisphenol studies
- to culture MCF-7 cells for experimental treatments with hormone
- to maintain zebrafish pineal glands for action spectrum analysis
Caratteristiche e vantaggi
Contains high concentrations of amino acids so the medium resembles the protein composition of cultured mammalian cells
Quantità
Formulated to contain 9.3 grams of powder per liter of medium.
Ricostituzione
Supplement with 0.292 g/L L-glutamine, 2.2 g/L sodium bicarbonate.
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Codice della classe di stoccaggio
13 - Non Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
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Protocolli
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
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