C2181

Anti-Mouse IgG (whole molecule) F(ab′)₂ fragment–Cy3 antibody produced in sheep

affinity isolated antibody, buffered aqueous solution

• Sinonimo/i: Cy3 Anti-Mouse IgG, Cy3 Mouse IgG, Sheep Anti-Mouse IgG
• Numero MDL: MFCD02179333
• Codice UNSPSC: 12352203
• NACRES: NA.46

Proprietà

• Origine biologica: sheep
• Coniugato: CY3 conjugate
• Forma dell’anticorpo: affinity isolated antibody
• Tipo di anticorpo: secondary antibodies
• Clone: polyclonal
• Forma fisica: buffered aqueous solution
• Reattività contro le specie: mouse
• tecniche: immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100
• Condizioni di spedizione: wet ice
• Temperatura di conservazione: 2-8°C
• modifica post-traduzionali bersaglio: unmodified

Descrizione

Descrizione generale

Immunoglobulins (Igs) belong to the immunoglobulin super-family. IgG is an abundant protein in human serum. The four classes of IgG include IgG1, IgG2, IgG3 and IgG4.. The IgG heavy chain region is mapped to human chromosome 14. Igs have two heavy (H) and two light (L) chains, held together by disulfide linkages. The heavy chain has one variable N-terminal region and three to four constant (CH1-CH4) C-terminal regions. The L chain comprises of one variable N-terminal region and a constant C-terminal region.

Applicazioni

Anti-Mouse IgG (whole molecule) F(ab′)2 fragment-Cy3 antibody produced in sheep was used for BrdU staining of frozen quail muscle cross sections at a dilution of 1:200 to analyze activated, proliferating muscle precursor cells.

Anti-Mouse IgG (whole molecule) F(ab′)₂ fragment−Cy3 antibody produced in sheep has been used:

• in immunolabeling of Hela cells
• as secondary antibody in immunocytochemistry of dendritic cells
• as secondary antibody in immunofluorescence analysis of mesenchymal stem cells
• as secondary antibody in immunofluorescence staining keratinocyte cell lines
• in immunohistochemistry of articular cartilage

The product binds to all mouse Igs and is useful when trying to avoid background staining due to the presence of Fc receptors.

Azioni biochim/fisiol

Digestion of IgG by papain results in the generation of fragment antigen binding (Fab). Pepsin digestion of IgG results in fragment crystallizable (Fc). The Fc region of IgG antibody has enormous therapeutic potential and is exploited for the development of therapeutic antibodies.

IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. The coupling of Cy3 to Anti-Mouse IgG (whole molecule) F(ab′)2 fragment antibody allows for the visualization of protein by fluorescent microscopy.

Altre note

Antibody adsorbed with human serum proteins.

Stato fisico

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Nota sulla preparazione

Adsorbed to reduce background staining with human samples.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): nwg
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable