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H7039

Sigma-Aldrich

3-Hydroxy-3-methylglutaryl-CoA reductase

solution

Sinonimo/i:

HMGCoA Reductase, HMGR

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About This Item

Classificazione EC (Enzyme Commission):
1.1.1.34 (BRENDA, IUBMB)
Codice UNSPSC:
12352204
NACRES:
NA.54

Saggio

≥90% (SDS-PAGE)

Livello qualitativo

200

Forma fisica

solution

Attività specifica

2-8 units/mg protein

PM

~76 kDa (for the fusion protein)

N° accesso UniProt

P04035

Condizioni di spedizione

dry ice

Temperatura di conservazione

−70°C

Informazioni sul gene

human ... HMGCR(3156)

Applicazioni

3-Hydroxy-3-methylglutaryl-CoA reductase human has been used:
  • in anti-HMGCR enzyme linked immunosorbent assay (ELISA)
  • as an antigen to measure anti-HMGCR antibodies by blot analysis
  • in HMG-CoA reductase activity assays
  • as an antigen to compare in-house antigen via sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE)/western blot

Azioni biochim/fisiol

3-Hydroxy-3-methylglutaryl-CoA reductase/HMGCR serves as a rate limiting enzyme in mevalonate pathway. It also controls the oncogenic Hippo transducers TAZ/YAP. HMGCR also perform as a genetic modifier for Alzheimer′s disease (AD).
Hydroxy-3-methylglutaryl-CoA reductase catalyzes the committed step in cholesterol biosynthesis.

Proprietà fisiche

Consists of the HMG CoA reductase catalytic domain fused to GST protein.

Definizione di unità

One unit will convert 1.0 μmole of NADPH to NADP+ per minute at 37 °C.

Stato fisico

Supplied as a solution containing 250 μg protein in 50 mM Tris pH 7.5, with 5 mM DTT, 1:200 Protease Inhibitor Cocktail (Catalog Number P8340) and 50% (w/v) glycerol.

Inibitore

N° Catalogo
Descrizione
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Substrato

N° Catalogo
Descrizione
Determinazione del prezzo

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Cocktail di inibitori delle proteasi for use with mammalian cell and tissue extracts, DMSO solution

Sigma-Aldrich

P8340

Cocktail di inibitori delle proteasi

Analysis of autoantibodies to 3-hydroxy-3-methylglutaryl-coenzyme A reductase using different technologies
Musset L, et al.
Journal of immunology research, 2014 (2014)
ChemVassa: a new method for identifying small molecule hits in drug discovery
Moldover B, et al.
The Open Medicinal Chemistry Journal, 6, 29-29 (2012)
OP0009 Distinctive Immunofluorescence Pattern in Statin-Associated Autoimmune Myopathy with Anti-HMGCR Autoantibodies
Alvarado-Cardenas M, et al.
Annals of the Rheumatic Diseases, 75, 56-56 (2016)
Myopathy with anti-HMGCR antibodies Perimysium and myofiber pathology
Alshehri A, et al.
Neurology? neuroimmunology & neuroinflammation, 2(4), e124-e124 (2015)
Marie Hudson et al.
Medicine, 95(44), e5280-e5280 (2016-11-20)
The objective of this study was to investigate the frequency of autoantibodies to hydroxymethylglutaryl coenzyme A reductase (HMGCR) in systemic sclerosis (SSc) and associations with inflammatory myositis and statin exposure.This was a cross-sectional, multicenter study of 306 subjects from the
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Articoli

Cholesterol Biosynthesis

Biosynthesis of cholesterol generally takes place in the endoplasmic reticulum of hepatic cells and begins with acetyl- CoA, which is mainly derived from an oxidation reaction in the mitochondria. Acetyl-CoA and acetoacetyl-CoA are converted to 3-hydroxy- 3-methylglutaryl-CoA (HMG-CoA) by HMG-CoA synthase.

Antilipemic Agents

Randomized controlled clinical studies have suggested 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase inhibitors (statins) are effective in both primary and secondary prevention of cardiovascular disease (CVD) events.

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