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H0393

Sigma-Aldrich

Hirudin

recombinant, expressed in unspecified host, ≥7,000 ATU/mg protein, powder

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About This Item

Numero CAS:
8001-27-2
Numero CE:
232-279-1
Numero MDL:
MFCD00145679
Codice UNSPSC:
12352202
NACRES:
NA.77

product name

Hirudin, recombinant, expressed in unspecified host, ≥7,000 ATU/mg protein (ATU = antithrombin units)

Ricombinante

expressed in unspecified host

Livello qualitativo

200

Forma fisica

powder

Attività specifica

≥7,000 ATU/mg protein (ATU = antithrombin units)

PM

~7 kDa

Solubilità

pyridine: soluble
water: soluble

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

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Applicazioni

Antithrombotic efficacy by parenteral route is substantially prolonged by incorporation into cationic liposomes, which act as a sort of sustained-release device.

Proprietà fisiche

The anticoagulant hirudin is a ~7 kDa acidic protein containing 65 amino acid residues. It occurs naturally in leeches (Hirudo medicinalis). It is the most potent natural inhibitor of both soluble and clot-bound thrombin. Hirudin binds thrombin with high affinity and covers more than 20% of the surface area of thrombin, occluding both the active site and exosite I (fibrinogen and PAR recognition site). This coverage blocks thrombus growth and platelet activation. Hirudin is not metabolized in the bloodstream of humans and is eliminated unchanged via kidney filtration. Native hirudin contains a sulfated tyrosyl residue (Tyr63), three disulfide bridges, and a high proportion of dicarboxylic acids. Hirudin is not glycosylated, and lacks tryptophan, arginine, and methionine residues. At least 20 isoforms have been identified and sequenced.
This recombinant hirudin product corresponds to the HV-1 variant sequence, with the exception that the Tyr63 residue is not sulfated.

Definizione di unità

One antithrombin unit (ATU) will neutralize one NIH unit of thrombin at 37 °C, based on direct comparison to an NIH thrombin reference standard.

Ricostituzione

Hirudin is soluble in water. The literature cites the use of "Dilution Fluid II" (35.7 mM acetic acid, 35.7 mM sodium diethyl barbiturate, 0.85% NaCl, 1% bovine serum albumin, and 0.5% PEG, pH 7.4) to dissolve hirudin (500 ATU/mL) and thrombin. Hirudin is reported to be soluble in pyridine, but practically insoluble in alcohol, ether, acetone, or benzene.

Risultati analitici

Protein determined by Lowry.

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

dust mask type N95 (US), Eyeshields, Gloves

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Hirudin as an inhibitor of thrombin.
Markwardt, F.
Methods in Enzymology, 19, 924-932 (1970)
[The reaction between hirudin and thrombin].
F MARKWARDT et al.
Hoppe-Seyler's Zeitschrift fur physiologische Chemie, 312(1-3), 85-98 (1958-01-01)
Hirudin and hirudin-based peptides.
S R Stone et al.
Methods in enzymology, 223, 312-336 (1993-01-01)
Serdar Duzgun et al.
Indian journal of plastic surgery : official publication of the Association of Plastic Surgeons of India, 47(1), 102-108 (2014-07-06)
The effect of recombinant hirudin, which is the most powerful antithrombotic agent, on flaps with venous insufficiency was investigated. Oedema and congestion are frequent on flaps, causing necrosis unpredictably. Venous insufficiency and thrombosis are experimentally and clinically more frequent than
R Bischoff et al.
Biochemistry, 32(2), 725-734 (1993-01-19)
Natural hirudin variant 2 with a lysine residue in position 47 (rHV2-Lys47) was produced in a genetically engineered strain of Saccharomyces cerevisiae as a secreted protein of 65 amino acids and purified to greater than 99% homogeneity. Only reversed-phase high-performance
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