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Documenti fondamentali

C7688

Sigma-Aldrich

Chaperonin 60 from Escherichia coli

>95% (SDS-PAGE), recombinant, expressed in E. coli overproducing strain, lyophilized powder

Sinonimo/i:

Cpn60, GroEL

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About This Item

Numero MDL:
MFCD00163256
Codice UNSPSC:
12352200
NACRES:
NA.32

Origine biologica

Escherichia coli

Livello qualitativo

200

Ricombinante

expressed in E. coli overproducing strain

Saggio

>95% (SDS-PAGE)

Stato

lyophilized powder

tecniche

electron microscopy: suitable
mass spectrometry (MS): suitable

Compatibilità

passes test (Refolding (w/GroES))

N° accesso UniProt

P10809

Temperatura di conservazione

2-8°C

Informazioni sul gene

human ... HSPD1(3329)

Descrizione generale

Research area: Cell Signaling

Chaperonin 60 (GroEL) and chaperonin 10 (GroES) belong to the ubiquitous family of heat-shock molecular chaperones found in prokaryotes and in eukaryotic organelles.

Applicazioni

Chaperonin 60 from Escherichia coli has been used:
  • in mass spectroscopy
  • in cryo-electron microscopy imaging as control for testing particle distribution
  • as standard in infrared spectrum measurements
  • as a protein sample for stability assessment and characterization studies using differential mobility analysis (DMA) and cryo-electron microscopy (cryo-EM).

Azioni biochim/fisiol

Apart from its role in facilitating protein folding, chaperonin 60 (Cpn60) serves as an extracellular signaling protein, showing functional similarities to certain proinflammatory cytokines. Furthermore, Cpn60 contributes to the inhibition of lipid accumulation and adipogenesis during the initial stages of cellular differentiation, thereby exerting anti-obesity effects.

Confezionamento

Package size based on protein content.

Stato fisico

Lyophilized powder containing Tris buffer salts, potassium chloride, magnesium chloride, dithiothreitol, and trehalose as stabilizer.

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)

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Certificati d'analisi (COA)

Lot/Batch Number
069M4115V
11190116
027M4094V
11160914
086M4092V

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Infrared irradiation in the collision cell of a hybrid tandem quadrupole/time-of-flight mass spectrometer for declustering and cleaning of nanoelectrosprayed protein complex ions
El-Faramawy A, et al.
Analytical Chemistry, 82(23), 9878-9884 (2010)
Self-assembled monolayers improve protein distribution on holey carbon cryo-EM supports
Meyerson JR, et al.
Scientific Reports, 4(2), 7084-7084 (2014)
Separating and visualising protein assemblies by means of preparative mass spectrometry and microscopy
Benesch JLP, et al.
Journal of Structural Biology, 172(2), 161-168 (2010)
Stéphane Erb et al.
Methods in molecular biology (Clifton, N.J.), 2247, 173-191 (2020-12-11)
By maintaining intact multi-protein complexes in the gas-phase, native mass spectrometry provides their molecular weight with very good accuracy compared to other methods (typically native PAGE or SEC-MALS) (Marcoux and Robinson, Structure 21:1541-1550, 2013). Besides, heterogeneous samples, in terms of
Zachary L VanAernum et al.
Nature protocols, 15(3), 1132-1157 (2020-02-02)
It is important to assess the identity and purity of proteins and protein complexes during and after protein purification to ensure that samples are of sufficient quality for further biochemical and structural characterization, as well as for use in consumer

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