G3153
β-Galactosidase from Escherichia coli
lyophilized powder, ≥500 units/mg protein
Sinonimo/i:
β-D-Galactoside galactohydrolase, Lactase
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About This Item
Prodotti consigliati
Ricombinante
expressed in E. coli
Livello qualitativo
Stato
lyophilized powder
Attività specifica
≥500 units/mg protein
PM
465 kDa
Temperatura di conservazione
−20°C
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Descrizione generale
β-galactosidase is a 465 kDa tetrameric protein. It is coded by the LacZ gene.
Applicazioni
β-Galactosidase from Escherichia coli has been used to produce biocatalytic Ca-alginate beads on a B-390 encapsulator (BUCHI). It has been used to prepare the source droplet solution and also as the cargo for Pep-1 (KETWWETWWTEWSQPKKKRKV-cysteamide).
β-Galactosidase may be used for derivatization, such as an enzyme label for IgG, without prior dialysis or gel filtration.
Azioni biochim/fisiol
β-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop.
β-galactosidase is considered as a reporter enzyme in applications involving gene expression regulation, analysis of protein function/structure and target gene expression.
Confezionamento
Package size based on protein content
Proprietà fisiche
Tetramer molecular weight 465 kDa (subunits 116.3 kDa each)
Definizione di unità
One unit will hydrolyze 1.0 μmole of o-nitrophenyl β-D-galactopyranoside to o-nitrophenol and D-galactose per min at pH 7.3 at 37 °C.
Stato fisico
Stabilized with phosphate buffer and sucrose
Inibitore
Substrato
N° Catalogo
Descrizione
Determinazione del prezzo
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
Eyeshields, Gloves, type N95 (US)
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