F2756

Fluorescein di(β-D-galactopyranoside)

• Sinonimo/i: FDG
• Formula empirica (notazione di Hill): C₃₂H₃₂O₁₅
• Numero CAS: 17817-20-8
• Peso molecolare: 656.59
• Numero MDL: MFCD00077344
• Codice UNSPSC: 12352201
• ID PubChem: 24894829
• NACRES: NC.07

Proprietà

• Saggio: ≥98% (HPLC)
• Forma fisica: powder
• Solubilità: methanol: 20 mg/mL, clear, yellow
• Temperatura di conservazione: −20°C
• Stringa SMILE: OC[C@H]1O[C@@H](Oc2ccc3c(Oc4cc(O[C@@H]5O[C@H](CO)[C@H](O)[C@H](O)[C@H]5O)ccc4C36OC(=O)c7ccccc67)c2)[C@H](O)[C@@H](O)[C@H]1O
• InChI: 1S/C32H32O15/c33-11-21-23(35)25(37)27(39)30(45-21)42-13-5-7-17-19(9-13)44-20-10-14(43-31-28(40)26(38)24(36)22(12-34)46-31)6-8-18(20)32(17)16-4-2-1-3-15(16)29(41)47-32/h1-10,21-28,30-31,33-40H,11-12H2/t21-,22-,23+,24+,25+,26+,27-,28-,30-,31-/m1/s1; ZTOBILYWTYHOJB-WBCGDKOGSA-N

Descrizione

Applicazioni

Fluorescein di(β-D-galactopyranoside) (FDG) has been used for the detection of gene expression via FACS (fluorescence-activated cell sorting).

Fluorescein di(β-D-galactopyranoside) has been used as a substrate in β-galactosidase assay.

Azioni biochim/fisiol

Fluorescein di (β-D-galactopyranoside) (FDG) results in fluorescence after being hydrolyzed to fluorescein by β-galactosidase. It gives fluorescence proportional to the enzymatic activity. This molecule is mainly required for the detection of gene expression in transfected cells when using β-galactosidase (Lac Z) as a reporter gene.

Substrati

Fluorogenic substrate for β-galactosidase

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, type N95 (US)