C7438

Chaperonin 10 from Escherichia coli

≥95.0% (SDS-PAGE), recombinant, expressed in E. coli overproducing strain, lyophilized powder

• Sinonimo/i: GroES
• Numero MDL: MFCD00163256
• Codice UNSPSC: 12352200
• NACRES: NA.32

Proprietà

• Origine biologica: Escherichia coli
• Livello qualitativo: 200
• Ricombinante: expressed in E. coli overproducing strain
• Saggio: ≥95.0% (SDS-PAGE)
• Stato: lyophilized powder
• tecniche: activity assay: suitable
• N° accesso UniProt: P61604
• Temperatura di conservazione: 2-8°C
• Informazioni sul gene: human ... HSPE1(3336)

Descrizione

Azioni biochim/fisiol

Chaperonin60 (GroEL) and chaperonin10 (GroES) belong to the ubiquitous family of heat-shock molecular chaperones found in prokaryotes and in eukaryotic organelles. The chaperonins assist the folding of nascent, organelle-imported or stress-destabilized polypeptides. In vitro, purified GroEL together with purified GroES in the presence of Mg-ATP facilitate refolding and reactivation of denatured proteins, e.g., the photosynthetic enzyme rubisco and the mitochondrial enzyme rhodanese.
The folding activity of a 1:1 molar mixture of GroEL and GroES was tested using urea-denatured rhodanese. At least 2-fold reactivation of rhodanese over the spontaneous reactivation was obtained.

Confezionamento

Package size based on protein content.

Stato fisico

Lyophilized powder containing Tris buffer salts, potassium chloride, dithiothreitol and trehalose as stabilizer.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, type N95 (US)