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A1602

Sigma-Aldrich

Amiloglucosidasi

ammonium sulfate suspension, ≥40 units/mg protein

Sinonimo/i:

1,4-α-D-glucan glucoidrolasi, Eso-1,4-α-glucosidasi, Glucoamilasi

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About This Item

Numero CAS:
9032-08-0
Classificazione EC (Enzyme Commission):
3.2.1.3 (BRENDA, IUBMB)
Numero MDL:
MFCD00081350
Codice UNSPSC:
12352204
NACRES:
NA.54

Forma fisica

ammonium sulfate suspension

Livello qualitativo

200

Attività specifica

≥40 units/mg protein

Condizioni di spedizione

wet ice

Temperatura di conservazione

2-8°C

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Descrizione generale

Amyloglucosidase, a disaccharide enzyme is a member of the alpha-glucosidase type. It is produced by several species of Aspergillus genus.

Applicazioni

Amyloglucosidase from Aspergillus niger is used to hydrolyze α-D-glucosides. It may be used in the brewing of beer and in the production of bread and juices. Amyloglucosidase, from Sigma, has been used to hydrolyze glycogen into glucose monomers in order to study lipid accumulation in skeletal muscle .
Amyloglucosidase from Aspergillus niger has been used to liberate free glucose in the Belgica antarctica larva homogenate. It has also been used in digestive enzyme mixture for starch digestion.

Azioni biochim/fisiol

Amyloglucosidase from Aspergillus niger is capable of hydrolyzing the α-D-(1-4), the α-D-(1-6), and the α-D-(1-3) glucosidic bonds of oligosaccharides .

Definizione di unità

One unit will liberate 1.0 mg of glucose from starch in 3 min at pH 4.5 at 55 °C.

Stato fisico

Suspension in 3.2 M (NH4)2SO4 solution, pH approx. 6.0

Altre note

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificati d'analisi (COA)

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Articoli

How to Work with Enzymes Supplied as Ammonium Sulfate Suspensions

Instructions for working with enzymes supplied as ammonium sulfate suspensions

Protocolli

Enzymatic Assay of Amyloglucosidase (EC 3.2.1.3)

This procedure may be used for the determination of Amyloglucosidase activity using starch as the substrate.

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