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ROAMYGLL

Roche

Amyloglucosidase

from Aspergillus niger

Sinonimo/i:

Aspergillus niger, disaccharidase-type a-glucosidase

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About This Item

Classificazione EC (Enzyme Commission):
Codice UNSPSC:
12352204

Origine biologica

Aspergillus niger

Livello qualitativo

Stato

lyophilized

Attività specifica

6 U/mg (approximately 6 U/mg lyophilizate at +25°C with glycogen as the substrate)
~6 units/mg protein (At 25 °C with glycogen as the substrate.)

PM

97 kDa

Confezionamento

pkg of 3,500 U (11202367001)
pkg of 500 U (11202332001)

Produttore/marchio commerciale

Roche

pH ottimale

4.6-4.8

Temperatura di conservazione

2-8°C

Descrizione generale

Amyloglucosidase, a 1,4-α-D-glucan glucohydrolase, is a disaccharidase–type α-glucosidase. It is synthesized by several Aspergillus genus species. This exo-enzyme is one of the major industrial enzymes. The stability of amyloglucosidase can be increased by immobilization.

Specificità

Cleaves terminal glucoses that are α1,4- or α1,6-linked to an oligo- or polysaccharide of multiple glucose units. The product is D-glucose.

Applicazioni

Amyloglucosidase from Aspergillus niger is used for the determination of starch.

Azioni biochim/fisiol

Amyloglucosidase from Aspergillus niger is capable of hydrolyzing the α-D-(1-4) and α-(1,6) glucosidic bonds of starch and malto-oligosaccharides. Amyloglucosidase is an extracellular enzyme that converts starch to dextrins and glucose. The enzyme is used in the starch-processing industry in the commercial production of D-glucose from corn syrup.

Nota sulla preparazione

Working concentration: Working concentration of conjugate depends on application and substrate.

The following concentrations should be taken as a guideline:
  • Western blot: 1 to 10 μg/ml


Working solution: Tris-buffered saline containing 0.1% Tween 20.

Storage conditions (working solution): 2 to 8 °C

Altre note

For life science research only. Not for use in diagnostic procedures.

Pittogrammi

Health hazard

Avvertenze

Danger

Indicazioni di pericolo

Classi di pericolo

Resp. Sens. 1

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

does not flash

Punto d’infiammabilità (°C)

does not flash


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High fructose corn syrup: production, uses and public health concerns.
Parker K, et al.
Biotechnology and Molecular Biology Reviews, 5, 71-78 (2010)
Simultaneous amyloglucosidase and exo-polygalacturonase production by Aspergillus niger using solid-state fermentation.
Costa, J et al.
Brazilian Archives of Biology and Technology, 50, 759-766 (2007)
High-potency amyloglucosidase-producing mold of the Aspergillus niger group.
Smiley K L, et al.
Applied Microbiology, 12(5), 455-455 (1964)
Nicholson N.
Biodiversity: New Leads for the Pharmaceutical and Agrochemical Industries (2000)
Juliann R Seebauer et al.
Journal of experimental botany, 61(2), 511-519 (2009-11-18)
The relative role of the maternal source and the filial sink in controlling the composition of maize (Zea mays L.) kernels is unclear and may be influenced by the genotype and the N supply. The objective of this study was

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