TRYPSEQM-RO
Roche
Trypsin Sequencing Grade, modified
from bovine pancreas
Sinonimo/i:
Trypsin
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About This Item
Prodotti consigliati
Origine biologica
bovine pancreas
Livello qualitativo
Grado
protein sequencing grade
Forma fisica
lyophilized (salt-free)
PM
24.000 g/mol
Confezionamento
pkg of 4 × 100 μg (11418033001)
pkg of 4 × 25 μg (11418025001)
Produttore/marchio commerciale
Roche
Condizioni di stoccaggio
(Keep container tightly closed in a dry and well-ventilated place.)
Concentrazione
0.01-0.2 % (w/w)
tecniche
protein sequencing: suitable
Impurezze
Chymotrypsin
Colore
white
pH ottimale
8.0
Solubilità
10 g/L
Compatibilità
suitable for protein modification
N° accesso UniProt
applicazioni
life science and biopharma
Attività estranea
Contaminating activities corresponds
Chymotrypsin , contains
Temperatura di conservazione
2-8°C
Informazioni sul gene
cow ... PRSS1(780933)
Descrizione generale
Trypsin Sequencing Grade, modified, is isolated from bovine pancreas as a highly purified and specific protease, and subsequently modified.
Trypsin is a highly efficient and specific protease widely used in proteomics for protein digestion. It produces short peptides with specific characteristics that are compatible with current separation and identification methods such as liquid chromatography, mass spectrometry (MS).
Inhibitors: TLCK, DFP, PMSF, leupeptin, soybean trypsin inhibitor, trypsin inhibitor from hen egg, aprotinin, α2-macroglobulin,α1-antitrypsin, APMSF, and antipain.
Trypsin is a highly efficient and specific protease widely used in proteomics for protein digestion. It produces short peptides with specific characteristics that are compatible with current separation and identification methods such as liquid chromatography, mass spectrometry (MS).
Inhibitors: TLCK, DFP, PMSF, leupeptin, soybean trypsin inhibitor, trypsin inhibitor from hen egg, aprotinin, α2-macroglobulin,α1-antitrypsin, APMSF, and antipain.
Specificità
Trypsin is a serine endopeptidase. At pH 7.5–9, it specifically hydrolyzes proteins and peptide bonds C-terminally of Iysine and arginine. Amide and ester bonds of Arg and Lys are also cleaved. The specificity of Trypsin Sequencing Grade, modified, is verified with the oxidized B-chain of insulin (insulin Box) as a substrate. High concentrations of Trypsin Sequencing Grade, modified, one part by weight enzyme with 9 parts by weight insulin Box, are incubated for 18 hours to detect traces ofchymotrypsin impurities.
Applicazioni
Use Trypsin Sequencing Grade, modified, to generate glycopeptides from purified glycoproteins.
It is used for:
It is used for:
- Protein-structure elucidation
- Tryptic mapping
- Fingerprinting analysis
- Sequence analysis
- Translocation studies
- Protein identification
- Protein digestion during lipoprotein preparation for liquid chromatography-tandem mass spectrometry (LC-MS/MS)
Qualità
Purity: Free of impurities that may interfere with the separation of peptides in reversed-phase HPLC.
Nota sulla preparazione
Working concentration: 1/100 to 1/5 of the protein by weight
Storage conditions (working solution): -15 to -25 °C
Trypsin Sequencing Grade, modified, is more resistant to autolysis, even at pH values in the neutral and weakly basic range. The enzyme can be used in high concentrations.
A solution in 1% acetic acid or 1 mM HCI can be used for up to one week when stored at 2 to 8° C. Stored in aliquots at -15 to -25 °C, the solution is stable for at least one year without loss of activity.
Storage conditions (working solution): -15 to -25 °C
Trypsin Sequencing Grade, modified, is more resistant to autolysis, even at pH values in the neutral and weakly basic range. The enzyme can be used in high concentrations.
A solution in 1% acetic acid or 1 mM HCI can be used for up to one week when stored at 2 to 8° C. Stored in aliquots at -15 to -25 °C, the solution is stable for at least one year without loss of activity.
Stoccaggio e stabilità
Store dry
Altre note
For life science research only. Not for use in diagnostic procedures.
Avvertenze
Danger
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Organi bersaglio
Respiratory system
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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