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Key Documents

RTRYP-RO

Roche

Trypsin recombinant, Proteomics Grade

recombinant enzyme expressed in Pichia pastoris

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About This Item

Classificazione EC (Enzyme Commission):
Codice UNSPSC:
12352204
NACRES:
NA.21

Ricombinante

expressed in Pichia pastoris

Livello qualitativo

Saggio

95% (gel filtration)

Forma fisica

lyophilized

Attività specifica

≥150 units/mg protein

Confezionamento

pkg of 4 × 100 μg (03708969001)
pkg of 4 × 25 μg (03708985001)

Produttore/marchio commerciale

Roche

Temperatura di conservazione

−20°C

Descrizione generale

This trypsin preparation is generated from the recombinant Pichia pastoris strain and is, thus, free of chymotrypsin. Trypsin recombinant, Proteomics Grade, exhibits high specific activity.

Specificità

Serine endopeptidase, hydrolyzing specifically proteins and peptides at the carboxy side of the basic amino acids Arg and Lys. Amide and ester bonds of Arg and Lys are also cleaved.

Applicazioni

Trypsin recombinant, Proteomics Grade, is used to specifically digest proteins separated by 2D gel electrophoresis or liquid chromatographic methods during sample preparation for mass spectrometry.

Nota sulla preparazione

Stabilizers: Ca2+ in mM concentration range
Inhibitors: TLCK, DFP, PMSF, leupeptin, soybean trypsin inhibitor, trypsin inhibitor from hen egg, aprotinin, α2-macroglobulin, α1-antitrypsin, APMSF, and antipain.
Storage conditions (working solution): -15 to -25°C
The reconstituted solution is stable for 1 month at -15 to -25°C. Avoid repeated freezing/thawing!

Ricostituzione

Following reconstitution in 10 mM HCl, Trypsin, recombinant, proteomics grade is stable at -15 to -25 °C for up to one month. After first reconstitution the product must be stored in appropriate aliquots to avoid repeated freezing and thawing.

Altre note

For life science research only. Not for use in diagnostic procedures.

Pittogrammi

Exclamation markHealth hazard

Avvertenze

Danger

Indicazioni di pericolo

Classi di pericolo

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Organi bersaglio

Respiratory system

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

does not flash

Punto d’infiammabilità (°C)

does not flash


Certificati d'analisi (COA)

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A Acera et al.
Eye (London, England), 25(9), 1225-1233 (2011-06-28)
To analyze tear protein profile variations in patients with keratoconus (KC) and to compare them with those of control subjects. Tears from 12 normal subjects and 12 patients with KC were analyzed by two-dimensional gel electrophoresis (2-DE) and liquid chromatography-mass
Alexander Schmidt et al.
Methods in molecular biology (Clifton, N.J.), 492, 21-38 (2009-02-26)
Proteomics may be defined as the systematic analysis of proteins expressed in a given organism (Electrophoresis 16:1090-1094, 1995). Important technical innovations in mass spectrometry (MS), protein identification methods, and database annotation, over the past decade, now make it possible to

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