10874477001
Roche
Plasminogen
from human serum
Sinonimo/i:
plasminogen
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About This Item
Codice UNSPSC:
12352204
Prodotti consigliati
Origine biologica
human serum
Livello qualitativo
Forma fisica
lyophilized
Attività specifica
~11 units/mg protein (activated with streptokinase at 37 °C and pH 7.4 with Chromozym PL as the substrate.)
PM
90-94 kDa
Confezionamento
pkg of 20 U
Produttore/marchio commerciale
Roche
tecniche
activity assay: suitable
applicazioni
life science and biopharma
Condizioni di spedizione
wet ice
Descrizione generale
Plasminogen is a profibrinolysin from human serum. Plasminogen, the proenzyme of the fibrinolytically active enzyme plasmin, is a single chain glycoprotein. Various isoelectric forms exist and can be separated by means of isoelectric focusing (IEF). The lyophilizate is stabilized with bovine serum albumin (BSA).
Applicazioni
Plasminogen is used for the determination of the activity of plasminogen activators. The enzyme is a substrate for tissue plasminogen activator (t-PA) in the t-PA test and a reference substance for t-PA therapy. It is used as the in-process control in the production of t-PA.
Caratteristiche e vantaggi
Contents
Lyophilizate, stabilized with bovine serum albumin.
Lyophilizate, stabilized with bovine serum albumin.
Qualità
Contaminants: <0.05% plasmin; the preparation does not contain 6-aminohexanoic acid
Sequenza
Native plasminogen (glu-plasminogen) has a N-terminal glutamic acid group. Partial plasma proteolysis gives rise to a molecule with N-terminal lysine (lys-plasminogen). Cleavage with elastase results in val-plasminogen. The complete amino acid sequence contains 790 amino acids. Roche Applied Science supplies glu-plasminogen.
Nota sulla preparazione
Stabilizers: stabilized with BSA
Working solution: Solvent is recommended in 20 mM sodium phosphate buffer, pH 7.5, 100 mM NaCl.
Storage conditions (working solution): -15 to -25 °C
A solution in 20 mM sodium phosphate buffer, pH ca. 7.5, 100 mM NaCl is stable for several months without loss of activity when stored at -15 to -25 °C.
Note:
Plasminogen is susceptible to autolysis, which may result in activation. Contact with the skin or other contamination (e.g., by microorganisms), is therefore to be avoided.
Working solution: Solvent is recommended in 20 mM sodium phosphate buffer, pH 7.5, 100 mM NaCl.
Storage conditions (working solution): -15 to -25 °C
A solution in 20 mM sodium phosphate buffer, pH ca. 7.5, 100 mM NaCl is stable for several months without loss of activity when stored at -15 to -25 °C.
Note:
Plasminogen is susceptible to autolysis, which may result in activation. Contact with the skin or other contamination (e.g., by microorganisms), is therefore to be avoided.
Stoccaggio e stabilità
Store at 2 to 8 °C. (Store dry!)
Altre note
For life science research only. Not for use in diagnostic procedures.
Avvertenze
Warning
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
STOT RE 2
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 2
Punto d’infiammabilità (°F)
does not flash
Punto d’infiammabilità (°C)
does not flash
Certificati d'analisi (COA)
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I clienti hanno visto anche
Andrea Schweinitz et al.
The Journal of biological chemistry, 279(32), 33613-33622 (2004-05-20)
The serine protease urokinase-type plasminogen activator (uPA) interacts with a specific receptor (uPAR) on the surface of various cell types, including tumor cells, and plays a crucial role in pericellular proteolysis. High levels of uPA and uPAR often correlate with
Claudine Ferland et al.
Journal of immunology (Baltimore, Md. : 1950), 173(7), 4417-4424 (2004-09-24)
Increased eosinophil counts are a major feature of asthmatic airways. Eosinophil recruitment requires migration through epithelium and tissue extracellular matrix by activation of proteases. We assessed the capacity of IL-16, a CD4(+) cell chemotactic factor, to induce migration of eosinophils
H Roger Lijnen
Biochemistry. Biokhimiia, 67(1), 92-98 (2002-02-14)
Several molecular interactions between the matrix metalloproteinase (MMP) and the plasminogen/plasmin (fibrinolytic) system may affect cellular fibrinolysis. MMP-3 (stromelysin-1) specifically hydrolyzes urokinase (u-PA), yielding a 17 kD NH2-terminal fragment containing the functionally intact receptor (u-PAR)-binding sequence and a 32 kD
A Krüger et al.
Cancer gene therapy, 7(2), 292-299 (2000-04-19)
The serine protease urokinase-type plasminogen activator, uPA, when bound to its specific receptor, uPAR (CD87), plays a significant role in tumor cell invasion and metastasis. In breast cancer, enhanced uPA antigen in the primary tumor is correlated with poor prognosis
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