612200-M

Tissue Plasminogen Activator, Human

• Sinonimo/i: tPA
• Codice UNSPSC: 12352202

Proprietà

• Saggio: ≥95% (SDS-PAGE)
• Livello qualitativo: 100
• Forma fisica: lyophilized
• Attività specifica: ≥300 KU/mg protein
• Produttore/marchio commerciale: Calbiochem^®
• Condizioni di stoccaggio: OK to freeze
• Temperatura di conservazione: −20°C

Descrizione

Descrizione generale

Native tissue plasminogen activator from a human melanoma cell line. A serine protease that contains a single chain of 527 amino acid residues. Binds to fibrin via lysine binding sites at its amino terminus and activates bound plasminogen. Cleaves plasminogen to form active plasmin, the proteolytic enzyme that dissolves clots. Reported to be involved in neurite outgrowth, regeneration, and migration. Plays an important role in excitotoxin-induced neuronal degeneration. Dihydropyridine Ca²⁺ antagonist may increase levels of tPA.

Native tissue plasminogen activator from a human melanoma cell line. A serine protease that contains a single chain of 527 amino acids. Binds to fibrin via lysine binding sites at its amino terminus and activates bound plasminogen. Cleaves plasminogen to form active plasmin, the proteolytic enzyme that dissolves clots. Reported to be involved in neurite outgrowth, regeneration, and migration. Plays an important role in excitotoxin-induced neuronal degeneration.

Attenzione

Toxicity: Standard Handling (A)

Stato fisico

Lyophilized from 100 mM PBS, 3.5 mg/ml L-arginine, 0.001% Tween^®-80 Detergent.

Nota sulla preparazione

Prepared from cell line that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Ricostituzione

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Reconstitute with diH₂O to a final concentration of 1 mg/ml (e.g., 100 µg should be reconstituted in 100 µl).

Altre note

Kim, Y.H., et al. 1999. Science284, 647.
Tsirka, S.E., et al. 1995. Nature 377, 340.
Winther, K., et al. 1992. J. Cardiovasc. Pharmacol.19, S21.

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

TWEEN is a registered trademark of Croda International PLC

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 13 - Non Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable