04942078001
Roche
Dispase® II (neutral protease, grade II)
lyophilized, from bacterial, Roche, pkg of 5 × 1 g
Sinonimo/i:
dissociating enzyme, neutral protease, tissue dissociating
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About This Item
Prodotti consigliati
Origine biologica
bacterial
Livello qualitativo
Sterilità
non-sterile
Forma fisica
lyophilized
Attività specifica
≥0.8 units/mg protein (37 °C, casein as substrate, pH 7.5)
Confezionamento
pkg of 5 × 1 g
Produttore/marchio commerciale
Roche
pH ottimale
6.0-8.5
applicazioni
sample preparation
Descrizione generale
Dispase is a neutral, rapid-acting, gentle protease that separates intact epidermis from dermis. It also separates cultured intact epithelial cells from the substratum. It cleaves the basement membrane zone and leaves the epithelial cells intact.
Specificità
Dispase is a nonspecific metalloprotease.
Applicazioni
Dispase® is used for the preparation of cells from a wide variety of different tissues and organs. The enzyme has proven to be a rapid, effective, yet gentle agent for the disruption of extracellular matrix of tissues for releasing single cells for cell culture (primary cell culture) or harvesting cells already in culture to transfer to new substrate (secondary culture). Furthermore, it is used to prevent unwanted clumping of cells cultured in suspension.
Caratteristiche e vantaggi
- Rapid, effective, yet gentle agent that liberates cells with minimal cell damage
- Maintains cell membrane integrity
- Non-mammalian source - free of mycoplasma and animal virus contamination
- Extremely stable to influences of temperature, pH, and interference by serum components
- Easily inactivated by chelating agents or by dilution
- Delivers higher activity and convenience
Definizione di unità
One unit is the enzyme activity which liberates Folin-positive amino acids and peptides corresponding to 1 μmol (181 μg) tyrosine in 1 minute under assay conditions (pH 7.5, +37 °C).
A practical comparison of Roche units of Dispase® II with those cited in the Japanese literature (where Dispase® concentrations of 1,000 to 2,000 units/ml are not uncommon) suggests that one Roche unit equals approximately 600 Japanese units of dispase.
One unit of Roche Applied Science Dispase® equals 181 protease units (PU) measured as release of amino acids equivalent to 1 μg tyrosine per min and ml at pH 7.5 and +37 °C.
A practical comparison of Roche units of Dispase® II with those cited in the Japanese literature (where Dispase® concentrations of 1,000 to 2,000 units/ml are not uncommon) suggests that one Roche unit equals approximately 600 Japanese units of dispase.
One unit of Roche Applied Science Dispase® equals 181 protease units (PU) measured as release of amino acids equivalent to 1 μg tyrosine per min and ml at pH 7.5 and +37 °C.
Nota sulla preparazione
Activator: Optimal Ca2+ concentration is 2 mM. The enzyme preparations contain enough Ca2+ for optimal activity.
Inhibitors: EDTA, EGTA, Hg2+, other heavy metals. Dispase is not inhibited by serum.
Working concentration: 0.6 to 2.4 U/ml
Working solution: Preparation of stock and working solutions:
To produce a 10 mg/ml stock solution, dissolve the lyophilized Dispase® II enzyme in HEPES-buffered saline (50 mM HEPES/KOH pH 7.4, 150 mM NaCl). To produce the working solution, dilute the above stock solution with the culture medium for the isolated cells, at a final concentration of 0.6 to 2.4 U/ml. Note that concentrations higher than 2.4 U/ml are not recommended. For best results, filter the working solution using a 0.22 μm filter membrane.
Storage conditions (working solution): -15 to -25 °C
The reconstituted stock solution is stable at 2 to 8 °C for 2 weeks. For storage up to 2 months the stock solution should be frozen in aliquots. Avoid repeated freezing and thawing! The working solution diluted with PBS is stable at 2 to 8 °C for 3 days.
Inhibitors: EDTA, EGTA, Hg2+, other heavy metals. Dispase is not inhibited by serum.
Working concentration: 0.6 to 2.4 U/ml
Working solution: Preparation of stock and working solutions:
To produce a 10 mg/ml stock solution, dissolve the lyophilized Dispase® II enzyme in HEPES-buffered saline (50 mM HEPES/KOH pH 7.4, 150 mM NaCl). To produce the working solution, dilute the above stock solution with the culture medium for the isolated cells, at a final concentration of 0.6 to 2.4 U/ml. Note that concentrations higher than 2.4 U/ml are not recommended. For best results, filter the working solution using a 0.22 μm filter membrane.
Storage conditions (working solution): -15 to -25 °C
The reconstituted stock solution is stable at 2 to 8 °C for 2 weeks. For storage up to 2 months the stock solution should be frozen in aliquots. Avoid repeated freezing and thawing! The working solution diluted with PBS is stable at 2 to 8 °C for 3 days.
Stoccaggio e stabilità
Store at 2 to 8 °C. (Store dry!)
Altre note
For life science research only. Not for use in diagnostic procedures.
Note legali
Dispase is a registered trademark of Godo Shusei Co., Ltd.
Avvertenze
Danger
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Organi bersaglio
Respiratory system
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
does not flash
Punto d’infiammabilità (°C)
does not flash
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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