SCR139

Animal Free Collagenase/Dispase Blend I

Animal Free Collagenase/Dispase Blend I is a mixture of animal free collagenase, type B, and neutral protease/dispase, and is inteneded to be used when a more potent enzymatic dissociatation solution than collagnaease is desired.

• Sinonimo/i: MMP, Matrix metalloprotease, Dispase, Neutral Protease
• Codice UNSPSC: 12352204
• eCl@ss: 32160405
• NACRES: NA.54

Proprietà

• Livello qualitativo: 200
• caseinase activity: ≥1000 caseinase units/mg
• clostripain activity: ≤5.0 clostripain units/mg
• collagenase activity: ≥12,500 U/vial
• trypsin activity: ≤0.5 trypsin units/mg
• tecniche: cell culture | mammalian: suitable
• Condizioni di spedizione: wet ice

Descrizione

Descrizione generale

Crude collagenases are widely used in enzymatic primary cell isolation and tissue dissociation procedures. Most researchers employ either crude collagenase preparations such as Types I, II, III, and IV or chromatographically purified collagenase. Dispase is especially suitable for tissue disaggregation and subcultivation procedures since it does not damage cell membranes. EMD Millipore’s Animal Free Collagenase/Dispase Blend I is a mixture of animal free collagenase, type B, and neutral protease/dispase, and is inteneded to be used when a more potent enzymatic dissociatation solution than collagnaease is desired. The product is prepared and purified from cultures grown in medium completely devoid of animal based components and designed for bioprocessing applications where introduction of animal derived pathogens, such as BSE,TSE and other mammalian viruses, must be prevented.

Product Source: Isolated and purified from Clostridium histolyticum and Bacillus polymyxa cultures devoid of animal containing reagents.
This product is manufactured in an ISO9001:2008 certified enviroment.

Applicazioni

Animal Free Collagenase/Dispase Blend I is a mixture of animal free collagenase, type B, and neutral protease/dispase, and is inteneded to be used when a more potent enzymatic dissociatation solution than collagnaease is desired.

Research Category
Stem Cell Research

Research Sub Category
Enzymes & Biochemistry

Stato fisico

Product is diafiltered with water, filtered through a 0.22 micron membrane and lyophilized in vials.

Stoccaggio e stabilità

Store at 2-8°C. Further dilutions should be made into appropriate buffer and/or medium to which protein has been added, such as DMEM/1% Human Serum Albumin or equivalent.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Pittogrammi: GHS07
• Avvertenze: Warning
• Indicazioni di pericolo: H317
• Consigli di prudenza: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Classi di pericolo: Skin Sens. 1
• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable