IP04
Protein G Plus-Agarose Suspension
Protein G PLUS agarose suspension specifically formulated for immunoprecipitation.
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About This Item
Prodotti consigliati
Stato
slurry (Liquid)
contiene
≤0.1% sodium azide as preservative
Produttore/marchio commerciale
Calbiochem®
Condizioni di stoccaggio
do not freeze
tecniche
immunoprecipitation (IP): suitable
Compatibilità
suitable for immunoprecipitation
Condizioni di spedizione
wet ice
Temperatura di conservazione
2-8°C
Descrizione generale
Designed for immunoprecipitation applications. This product is blocked with BSA to reduce non-specific binding and cannot be used for purification; best for bovine, goat, human, and rat IgG and for mouse IgG1.
Protein G PLUS agarose suspension specifically formulated for immunoprecipitation.
Attenzione
Toxicity: Standard Handling (A)
Altre note
Agarose solution is supplied ready to use. Protein G Plus agrarose is blocked with BSA and should not be used for immunoglobulin purification or covalent cross-linking. For immunoprecipitation reactions 15 µl/µg primary antibody is recommended. Preclearing will minimize extra bands resulting from nonspecific precipitation. To preclear, add to the sample 20 µl agarose conjugate and 1 µg normal IgG from the same species as the immunoprecipitating antibody. When immunoblotting is used for detection, some secondary antibodies can react nonspecifically with BSA or other proteins present at high concentrations in the sample. This can be eliminated by reducing the concentration of secondary antibody.
Note legali
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
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