IP05
Protein G Plus/Protein A Agarose Suspension
Protein G PLUS/Protein A-Agarose mixture specifically formulated for immunoprecipitation.
Sinonimo/i:
Affinity resin, Protein G/Agarose
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About This Item
Codice UNSPSC:
41116133
NACRES:
NA.56
Prodotti consigliati
Forma fisica
slurry (Liquid)
contiene
≤0.1% sodium azide as preservative
Produttore/marchio commerciale
Calbiochem®
Condizioni di stoccaggio
do not freeze
tecniche
protein purification: suitable
Compatibilità
suitable for microbiology
Condizioni di spedizione
wet ice
Temperatura di conservazione
2-8°C
Descrizione generale
Designed for immunoprecipitation applications. This product is blocked with BSA to reduce non-specific binding and cannot be used for purification.
Protein G PLUS/Protein A-Agarose mixture specifically formulated for immunoprecipitation.
Applicazioni
Immunoprecipitation (see comments)
Attenzione
Toxicity: Standard Handling (A)
Stato fisico
33% slurry in PBS.
Altre note
Agarose solution is supplied ready to use. Protein G Plus/Protein A Agarose immunoprecipitation reagent is blocked with BSA and should not be used for immunoglobulin purification or covalent cross-linking. For immunoprecipitation reactions 15 µl of solution per µg primary antibody is recommended. Preclearing will minimize extra bands resulting from nonspecific precipitation. To preclear, add to the sample 20 µl of agarose conjugate and 1 µg of normal IgG from the same species as the immunoprecipitating antibody. When immunoblotting is used for detection, some secondary antibodies can react nonspecifically with BSA or other proteins present at high concentrations in the sample. This can be eliminated by reducing the concentration of secondary antibody.
Note legali
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
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