P2545
Protein A–Agarose
saline suspension
Sinonimo/i:
Protein A resin
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About This Item
Prodotti consigliati
Origine biologica
Staphylococcus aureus
Livello qualitativo
Stato
saline suspension
Grado di funzionalizzazione
~2 mg per mL
Attivazione matrice
cyanogen bromide
Gruppi immobilizzati alla matrice
amino
Braccio spaziatore
1 atom
Capacità
≥15 mg/mL binding capacity (human IgG)
Temperatura di conservazione
2-8°C
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Descrizione generale
Protein A is a 42-kDa protein from Staphylococcus aureus and has five immunoglobulin binding domains.
Applicazioni
Protein A−Agarose has been used in the immunoprecipitation
- of protein phosphatase and tensin homolog (PTEN) protein from S63 and NIH3T3 fibroblast cells
- of prolyl hydroxylase domain 2 N27 cells
- of Wnt3a and sonic hedgehog (Shh) in mouse fibroblast L-cells and human embryonic kidney (HEK) 293 cells
Protein A-agarose is used for affinity chromatography, antibody purification and characterization, and protein A, G and L resins. Protein A-agarose has been used to study the effects of protein A immunoadsorption in patients with chronic dilated cardiomyopathy as well as to study multiple sclerosis and gastric cancer.
Azioni biochim/fisiol
Protein A binds to Fcγ (the constant region) and Fab (antigen binding fragment) of IgG. Protein A agarose resin is commercially used for affinity based purifications.
Stato fisico
Suspension in 0.5 M NaCl containing preservative
Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Dispositivi di protezione individuale
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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