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524627

Millipore

Phosphatase Inhibitor Cocktail III

liquid, for the inhibition of serine/threonine and protein tyrosine phosphatases

Sinonimo/i:

Phosphatase Inhibitor Mix (Set III), Phosphatase inhibitor

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About This Item

Codice UNSPSC:
12352200
NACRES:
NA.77

product name

Phosphatase Inhibitor Cocktail Set III, Phosphatase Inhibitor Cocktail Set III is a ready to use cocktail of four phosphatase inhibitors for broad-spectrum inhibition of both serine/threonine and protein tyrosine phosphatases.

Forma fisica

liquid

Produttore/marchio commerciale

Calbiochem®

Condizioni di stoccaggio

OK to freeze

Condizioni di spedizione

wet ice

Temperatura di conservazione

−20°C

Descrizione generale

Phosphorylation and dephosphorylation regulate signal transduction processes involved in cellular activities such as cell division, proliferation, and apoptosis. Protein kinases add a phosphate group to specific amino acid residues (serine, threonine, or tyrosine) on target proteins, changing their conformation and interaction with ligands. Protein phosphatases remove the phosphate group, restoring the protein to its dephosphorylated state. When extracting phosphorylated proteins from cell and tissue lysates, it is crucial to maintain their phosphorylation status intact. Phosphatase Inhibitor Cocktail Set III is a cocktail of four phosphatase inhibitors for broad-spectrum inhibition of both serine/threonine and protein tyrosine phosphatases. Available as a 1 ml vial or as a set of five 1 ml vials.

Applicazioni

Phosphatase Inhibitor Cocktail Set III has been used:
  • as a supplement in lysis buffer to prepare protein lysates of whole testes for western blotting
  • to fortify ice-cold lysis radio-immunoprecipitation assay (RIPA) buffer to homogenize larval hindguts for western blot analysis
  • as a component in cell lysis buffer to extract total proteins for western blotting

Azioni biochim/fisiol

Cell permeable: no
Primary Target
Serine/threonine and protein tyrosine phosphatases
Product does not compete with ATP.
Reversible: no

Caratteristiche e vantaggi

  • Ready-to-use formulations for convenient use
  • High-quality products for consistent and reproducible performance,
  • Flexible options with a range of cocktails targeting different categories of phosphatases

Componenti

Each vial contains 1 ml of aqueous solution with the following components:
  • 50 mM Sodium Fluoride
  • 10 mM ß-Glycerophosphate (Cat. No. 35675)
  • 10 mM Sodium Pyrophosphate Decahydrate
  • 1 mM Sodium Orthovanadate

Attenzione

Toxicity: Toxic (F)

Stato fisico

In H₂O.

Ricostituzione

For long-term storage, aliquot and freeze (-20°C). Dilute 1:5 just prior to use.

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

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