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Key Documents

I3786

Sigma-Aldrich

Protease Inhibitor Cocktail

Animal-Free, DMSO solution, for the inhibition of serine, cysteine, aspartic proteases and aminopeptidases, for use with mammalian cell and tissue extract, DMSO solution

Sinonimo/i:

Protease inhibitor

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About This Item

Codice UNSPSC:
12352200
NACRES:
NA.77

product name

Protease Inhibitor Cocktail, Animal Component Free, for use with mammalian cell and tissue extract, DMSO solution

Livello qualitativo

Forma fisica

DMSO solution

Temperatura di conservazione

−20°C

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Descrizione generale

Components of this cocktail are of non-animal origin and no animal products were used in the component production process. All vessels and instruments used for the cocktail production are dedicated for animal component free production and have never encountered an animal product. Aprotinin used in this cocktail is a recombinant bovine protein expressed in plants (Nicotiana).
This protease inhibitor cocktail has been optimized and tested for mammalian cell and tissue extracts.

Specificità

Inhibits serine, cysteine, aspartic proteases, and aminopeptidases.

Applicazioni

Animal component free (ACF). Optimized and tested for general use. Can also be used for purification of histidine-tagged proteins, as chelating agents have been omitted.

Componenti

AEBSF
Aprotinin
Bestatin
E-64
Leupeptin
Pepstatin A

Stato fisico

The components in this ACF protease inhibitor cocktail do not originate from animal sources and can thus be used in the production of animal-free proteins.
This ACF cocktail is supplied as a ready-to-use solution in DMSO. One ml of the cocktail solution is recommended for the inhibition of endogenous enzymes found in 100 mL of lysate from 20 g (wet weight) of bovine liver, or 10 mL of cell lysate obtained from CHO cells at a cell density of 108 cells per mL.

Pittogrammi

Exclamation mark

Avvertenze

Warning

Indicazioni di pericolo

Classi di pericolo

Eye Irrit. 2 - Skin Irrit. 2

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

188.6 °F - closed cup

Punto d’infiammabilità (°C)

87 °C - closed cup


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