524629
Phosphatase Inhibitor Cocktail V
50X, liquid, for the inhibition of serine/threonine and tyrosine phosphatase
Sinonimo/i:
Phosphatase Inhibitor Mix Set V, Phosphatase inhibitor
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About This Item
Codice UNSPSC:
12352200
NACRES:
NA.77
Prodotti consigliati
product name
Phosphatase Inhibitor Cocktail Set V, 50X, Phosphatase Inhibitor Cocktail Set V is a 50X cocktail of four serine/threonine and tyrosine phosphatase inhibitors.
Forma fisica
liquid
Produttore/marchio commerciale
Calbiochem®
Condizioni di stoccaggio
OK to freeze
protect from light
Condizioni di spedizione
wet ice
Temperatura di conservazione
−20°C
Descrizione generale
A cocktail of four phosphatase inhibitors for the inhibition of both serine/threonine and protein tyrosine phosphatases. Available as 1 ml or as a set of five 1 ml vials.
Applicazioni
Phosphatase Inhibitor Cocktail Set V, 50X has been used:
- as a supplement in the lysis buffer to dissociate metabolically activated one (MMe) bone marrow macrophages (BMDMs) for Western blot
- in radio-immunoprecipitation assay (RIPA) modified buffer to incubate cultured human umbilical vein endothelial cells (HUVECs) for western blot
- in lysis buffer to lyse cultured HUVECs for immunoprecipitation
- in wash buffer to wash the protein complexes
- as a component in 4-(2-hydroxyethyl) piperazine-1-ethanesulfonic acid (HEPES) buffer to homogenize J774 cells to assess the effect of lipopolysaccharides (LPS) incubation on cytosolic phospholipase A2 (cPLA2) activity
Azioni biochim/fisiol
Protein phosphorylation and dephosphorylation play a crucial role in signal transduction, controlling various cellular processes such as cell division, proliferation, and apoptosis. Protein kinases add a phosphate group to specific serine, threonine, or tyrosine residues, which alters the protein′s conformation and its ability to interact with ligands. Protein phosphatases then remove the phosphate group, restoring the protein to its original dephosphorylated state. When extracting phosphorylated proteins from cell and tissue lysates, it is of utmost importance to preserve their phosphorylation state.
Caratteristiche e vantaggi
- Convenient: formulations are ready-to-use, making them easy to use.
- Consistent: high-quality formulations ensure reliable and reproducible performance.
- Flexible: the cocktails are designed to target different categories of phosphatases, offering versatility in experimentation.
Componenti
Each vial contains 1 ml of aqueous solution with the following components:
- 250 mM Sodium Fluoride
- 50 mM ß-Glycerophosphate (Cat. No. 35675)
- 50 mM Sodium Pyrophosphate Decahydrate
- 50 mM Sodium Orthovanadate
Attenzione
Toxicity: Toxic (F)
Stato fisico
In H₂O.
Ricostituzione
Following initial thaw, aliquot and freeze (-20°C) for long-term storage. Dilute 1:50 just prior to use.
Note legali
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Avvertenze
Warning
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Eye Irrit. 2
Codice della classe di stoccaggio
12 - Non Combustible Liquids
Classe di pericolosità dell'acqua (WGK)
WGK 2
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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