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一般說明
Neuroporter™ 转染试剂是经过优化的专利阳离子脂质体配方，毒性低，可用于向原代神经细胞、神经胶质细胞以及培养的神经细胞系之中高效递送DNA。Neuroporter™转染试剂盒可用于难转染原代神经细胞，可解决诸如细胞活力不足、转染效率低以及神经细胞退化等之前存在的问题。
應用
适于向原代神经细胞和培养的神经细胞系之中瞬时和稳定转染核酸。每一块6 cm细胞培养板可使用大约15-120 μl Neuroporter™的转染试剂和6-8 μg 的DNA（可应客户要求，以溶于独特的DNA稀释液的形式提供）。以下细胞已采用Neuroporter™转染试剂盒成功转染。
- C6胶质瘤细胞（人类）
- 皮质神经元细胞（大鼠原代细胞）
- 背根神经节(DRG)细胞（大鼠）
- NT2神经细胞（人前体细胞）
- NT神经元（人分化细胞）
- 室下区 (SVZ)细胞（小鼠）
- 白质细胞（小鼠）
特點和優勢
- 针对原代神经细胞、神经胶质细胞以及培养的神经细胞系进行了优化
- 具有超低的细胞毒性，不会造成神经细胞退化或树突戒断
- 可有效地递送原代神经细胞、神经胶质细胞以及培养的神经细胞系
- 相对于其他方法，使用更为轻松、快捷
- 兼容含血清和不含血清的转染实验
成分
1小瓶Neuroporter™ 转染试剂，为干燥的脂质体膜形式(T2823)
1.5 mL缓冲液 H9036
7.5 mL DNA稀释液D1941
1.5 mL缓冲液 H9036
7.5 mL DNA稀释液D1941
注意
注意：不要冻存。
原則
在无血清的条件下混合Neuroporter™转染试剂和DNA，即可形成稳定的非共价偶联复合物。该复合物性质稳定，可直接加入到细胞培养基中，并在培养基中与细胞膜融合，将DNA释放到细胞质中。注意：复合物的形成受到血清抑制，但一旦形成稳定的复合物，即便存在血清也无妨。
法律資訊
NeuroPorter is a trademark of Gene Therapy Systems, Inc.
儲存類別代碼
12 - Non Combustible Liquids
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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Transfection is the introduction of DNA, RNA, or proteins into eukaryotic cells and is used in research to study and modulate gene expression. Thus, transfection techniques and protocols serve as an analytical tool that facilitates the characterization of genetic functions, protein synthesis, cell growth and development.
This brief webinar provides an overview of what transfection is and the methods that are used to introduce DNA or RNA into eukaryotic cells.
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