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L3037

Sigma-Aldrich

Escort™ III 转染试剂

Lipid reagent for transfecting sensitive and primary cells

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About This Item

分類程式碼代碼:
41106502
NACRES:
NA.25

等級

for molecular biology

品質等級

200

形狀

liquid (aqueous solution)

用途

 mL sufficient for 250-1000 transfections

濃度

1 mg/mL

技術

transfection: suitable

儲存溫度

2-8°C

相关类别

一般說明

Escort™ III 是一种含有专有聚阳离子脂质和中性非转染脂质的独特制剂。 该脂质体形成化合物用于将核酸转染到原代细胞中。

應用

适用于瞬时和稳定地将核酸转染至培养的真核细胞中。 每个 6 cm 细胞培养板使用大约 2-8 μl Escort™ III 和 2μ g DNA。 方案优化带来了非常高效的转染。 使用 Escort ™III 已成功转染以下细胞：

A549
C2C12 肌管
心肌细胞（大鼠）
COS-7
成纤维细胞（大鼠）
生殖细胞（雄性大鼠）
肝细胞（大鼠和仓鼠）
HepG2
HeLa
Jurkat
角化细胞（人）
成肌细胞（小鼠和鹌鹑）
肌细胞（小鼠）
NIH3T3
PC-12
视网膜神经元（大鼠）
气管支气管细胞（绵羊）

特點和優勢

  • 适用于稳定和瞬时转染
  • 针对多种原代细胞进行了优化
  • 低毒性
  • 兼容血清和无血清转染方案
  • PC-12 细胞的理想选择

成分

Escort™ III 制剂：
1 mg/mL 总脂质，溶于水

请注意，Escort™ III 使用的脂质特性为保密信息。

注意

请勿冷冻。

原則

当 Escort™ III 在没有血清的情况下与 DNA 混合时，会形成一种稳定的复合物。 复合物是稳定的，可以直接添加到细胞培养基中，在培养基中它们与细胞膜融合，从而将 DNA 释放到细胞质中。 注意：血清会抑制复合物的形成，但是稳定的复合物一旦形成，血清的存在就不会有任何后果。

法律資訊

Escort is a trademark of Sigma-Aldrich Co. LLC

儲存類別代碼

10 - Combustible liquids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves

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Tomás C O'Riordan et al.
American journal of physiology. Regulatory, integrative and comparative physiology, 292(4), R1613-R1620 (2006-12-16)
The development and application of a methodology for measurement of oxygen within single mammalian cells are presented, which employ novel macromolecular near infrared (NIR) oxygen probes based on new metalloporphyrin dyes. The probes, which display optimal spectral characteristics and sensitivity
Patrick Orth et al.
Molecular biotechnology, 38(2), 137-144 (2008-01-26)
The aim of the present study was to evaluate the efficacy of novel nonviral gene delivery systems in cells of musculoskeletal origin. Primary cultures of lapine skeletal muscle cells, lapine articular chondrocytes, human cells from fibrous dysplasia and cell lines
Inmaculada Navarro-Lérida et al.
Journal of cell science, 117(Pt 9), 1687-1697 (2004-04-13)
Several cell types express inducible nitric oxide synthase (NOS2) in response to exogenous insults such as bacterial lipopolysaccharide (LPS) or proinflammatory cytokines. For instance, muscular cells treated with LPS and interferon gamma (IFN-gamma) respond by increasing the mRNA and protein
Lixian Liu et al.
Frontiers in cell and developmental biology, 9, 634242-634242 (2021-03-12)
The mitogen-inducible gene 6 (MIG6) is an adaptor protein widely expressed in vascular endothelial cells. However, it remains unknown thus far whether it plays a role in angiogenesis. Here, using comprehensive in vitro and in vivo model systems, we unveil
Thomas Iskratsch et al.
The Journal of cell biology, 191(6), 1159-1172 (2010-12-15)
Members of the formin family are important for actin filament nucleation and elongation. We have identified a novel striated muscle-specific splice variant of the formin FHOD3 that introduces a casein kinase 2 (CK2) phosphorylation site. The specific targeting of muscle

商品

Introduction to Cell Transfection

Transfection is the introduction of DNA, RNA, or proteins into eukaryotic cells and is used in research to study and modulate gene expression. Thus, transfection techniques and protocols serve as an analytical tool that facilitates the characterization of genetic functions, protein synthesis, cell growth and development.

How Transfection Works

This brief webinar provides an overview of what transfection is and the methods that are used to introduce DNA or RNA into eukaryotic cells.

实验方案

Lipid Transfection Optimization Protocol

Expert guidance on the best way to optimize your transfection experiments. Optimized protocols result in highest efficiency transfections.

Escort™ IV Transfection Reagent Protocol

Product manual provides detailed protocol for easy DNA transfection.

Escort™ III Transfection Reagent Protocol

The product bulletin providin detailed use protocol for easy DNA transfection.

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