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XTGHP-RO

Roche

X-tremeGENE™ HP DNA转染试剂

High-performance polymer reagent for transfecting many cell lines

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About This Item

分類程式碼代碼:
41106502
NACRES:
NA.55

等級

for molecular biology

品質等級

100

形狀

liquid (aqueous solution)

用途

 mL (suitable for 165 transfections)

包裝

pkg of 0.4 mL (06366244001)
pkg of 1.0 mL (06366236001)
pkg of 5 × 1 mL (06366546001)

製造商／商標名

Roche

技術

transfection: suitable

儲存溫度

−20°C

相关类别

一般說明

X-tremeGENE HP DNA转染试剂是一款多组分试剂，能够与DNA形成复合物，并将其递送至动物或昆虫细胞中。

特點和優勢

X-tremeGENE HP DNA转染试剂是一种高效转染试剂，可成功转染多种真核细胞，包括昆虫细胞和难转染细胞系（如Sf9、U-2 OS、MEF、MCF7、Hep G2、PC-3、HeLa、HT-29、HT-1080、K-562、RAW 264.7、Jurkat、PC-12和HCT 116）。

  • 简单易用的非脂质体试剂 无动物源性成分，室温稳定，经0.2 μm滤膜过滤，并且在有血清培养液中仍保持活性。
  • 高转染效率 对于原代细胞和使用其它试剂难转染的肿瘤细胞系有高转染效率。
  • 结果生理相关性好 使用细胞毒性低的试剂。
  • 满足高通量实验 实现简单一致的靶标评估。

品質

每一批次的X-tremeGENE HP DNA转染试剂都经过严格的质量控制流程测试，确保性能合规的标准性。在质量测试环节，首先使用X-tremeGENE HP DNA转染试剂（试剂DNA比为3:1μl/μg DNA）向细胞转染一个报告基因载体。再通过化学荧光检测法监测报告基因活性。最后使用一条标准曲线对每孔的重组蛋白总量进行定量，以确定产品达到规格。

外觀

本产品溶于80%乙醇中，经0.2 μm滤膜过滤。无人源或动物源成分。次数：使用标准步骤时，在96孔板内，1ml X-tremeGENE™ HP DNA转染试剂可以进行10,000次转染。

其他說明

仅用于生命科学研究。不可用于诊断。

法律資訊

X-tremeGENE is a trademark of Roche

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产品编号
说明
价格

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杜氏改良 Eagle 培养基 - 高葡萄糖, With 4500 mg/L glucose, L-glutamine, and sodium bicarbonate, without sodium pyruvate, liquid, sterile-filtered, suitable for cell culture

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RPMI-1640 培养基, With L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

NA0300

GenElute™ HP质粒大量制备试剂盒, sufficient for 10 purifications

NA0400

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PP2393

哺乳动物FLAG®标签蛋白载体套装, plasmid vectors for molecular cloning

XTG9-RO

X-tremeGENE™ 9 DNA Transfection Reagent, Polymer reagent for transfecting common cell lines

SITRAN-RO

X-tremeGENE™ siRNA 转染试剂, Polymer reagent for delivering siRNA to common cell lines

象形圖

FlameExclamation mark
GHS02,GHS07

訊號詞

Danger

危險聲明

H225 - H319

危險分類

Eye Irrit. 2 - Flam. Liq. 2

儲存類別代碼

3 - Flammable liquids

水污染物質分類（WGK）

WGK 1

閃點（°F）

334.4 °F

閃點（°C）

168 °C

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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Xin Yong et al.
Cancer letters, 374(2), 292-303 (2016-03-05)
Helicobacter pylori (H. pylori) infection is considered a major risk factor for gastric cancer. CagA behaves as a major bacterial oncoprotein playing a key role in H. pylori-induced tumorigenesis. Cancer stem cells (CSCs) are believed to possess the ability to initiate tumorigenesis
Isabelle Coupienne et al.
Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology, 10(12), 1868-1878 (2011-10-29)
Glioblastoma constitute the most frequent and deadliest brain tumors of astrocytic origin. They are resistant to all current therapies and are associated with a high rate of recurrence. Glioblastoma were previously shown to respond to treatments by 5-aminolevulinic acid (5-ALA)-based
Richard R W Brady et al.
Carcinogenesis, 32(7), 1069-1077 (2011-05-10)
Long-term aspirin or related non-steroidal anti-inflammatory drugs (NSAIDs) ingestion can protect against colorectal cancer (CRC). NSAIDs have a pro-apoptotic activity and we have shown that stimulation of the nuclear factor-kappaB (NF-κB) pathway is a key component of this pro-apoptotic effect.
Jihoon Kim et al.
Bioorganic & medicinal chemistry, 19(24), 7582-7589 (2011-11-11)
The signal transducer and activator of transcription 3 (STAT3) is constitutively activated in cancer cells. Therefore, blocking the aberrant activity of STAT3 in tumor cells is a validated therapeutic strategy. To discover novel inhibitors of STAT3 activity, we screened against
Ido D Weiss et al.
Molecular imaging and biology : MIB : the official publication of the Academy of Molecular Imaging, 14(1), 106-114 (2011-02-25)
Expression of CXCR4 in cancers has been correlated with poor prognosis and increased metastasis. Quantifying CXCR4 expression non-invasively might aid in prognostication and monitoring therapy. We evaluated a radiolabeled antagonist of CXCR4, ⁶⁴Cu-AMD3100, as a positron-emitting imaging agent. CXCR4-transfected or
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实验方案

Lentiviral Production Using X-tremeGENE HP Transfection Reagent

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X-tremeGENE HP DNA Transfection Reagent Protocol

Cell preparation for transfection Plate cells approx. 24 hours before transfection making sure cells are at optimal concentration (70 – 90 % confluency).

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