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等級
for molecular biology
形狀
liquid (aqueous solution)
包裝
pkg of 0.4 mL (08724105001)
pkg of 1.0 mL (08724121001)
pkg of 5 × 1.0 mL (08724156001)
製造商／商標名
Roche
技術
transfection: suitable
儲存溫度
−20°C
相关类别
一般說明
應用
- 设计用于转染广泛的真核细胞，许多不能被其他试剂很好转染的细胞系以及难以转染的细胞系，例如asK-562和HepG2。
- 可以成功用于多种应用，例如使用瞬时转染的细胞进行基因表达分析，生成稳定的细胞系，用于基因敲除研究的siRNA表达以及CRISPR基因编辑工具。
- 当足够数量的细胞被转染时，产生最小的细胞毒性或形态变化，无需在添加转染复合物后更换培养基。
- 适用于瞬时稳定转染。
- 存在或不存在血清时功能良好。
特點和優勢
- 在广泛的电池类型中达到峰值性能
- 适用于多个应用
- 质粒DNA、siRNA/miRNA和CRISPR/Cas9组分的高效递送
品質
外觀
其他說明
法律資訊
訊號詞
Danger
危險聲明
危險分類
Eye Irrit. 2 - Flam. Liq. 2
儲存類別代碼
3 - Flammable liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
61.7 °F
閃點（°C）
16.5 °C
其他客户在看
商品
MicroRNAs (miRNAs) are an endogenous class of RNA molecules comprising 19-25 nucleotides that regulate the suitability or transflational efficiency of mRNAs.
Automation is used for many applications to reduce variation caused by manual handling and to obtain reproducible results in high-throughput assays. High-throughput applications, such as knockdown studies or target screenings, often include cell transfection.
Small inhibitory RNAs (siRNAs) have become the focus of interest in many laboratories. For the first time, these molecules offer an easy way to knock down the expression of selected genes in mammalian cells without having to resort to classical gene knockout techniques.
Transfection is the introduction of DNA, RNA, or proteins into eukaryotic cells and is used in research to study and modulate gene expression. Thus, transfection techniques and protocols serve as an analytical tool that facilitates the characterization of genetic functions, protein synthesis, cell growth and development.
实验方案
Lentiviruses represent a powerful tool in research applications to transduce a wide range of cell types.
Plate cells approx. 24 hours before transfection making sure cells are at optimal concentration (70 – 90 % confluency).
X-tremeGENE™ siRNA Transfection Reagent Protocol & Troubleshooting
Transient co-transfection of plasmids is a method that is commonly employed for cellular protein-protein interaction studies, transcription factor studies, and gene knockdown studies using shRNA encoding plasmids.
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