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XTG9-RO

Roche

X-tremeGENE™ 9 DNA Transfection Reagent

Polymer reagent for transfecting common cell lines

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About This Item

分類程式碼代碼:
41106502
NACRES:
NA.55

等級

for molecular biology

品質等級

100

形狀

liquid (aqueous solution)

用途

 mL (suitable for 165 transfections)

包裝

pkg of 0.4 mL (06365779001)
pkg of 1.0 mL (06365787001)
pkg of 5 × 1.0 mL (06365809001)

製造商／商標名

Roche

技術

transfection: suitable

儲存溫度

2-8°C

相关类别

一般說明

X-tremeGENE 9 DNA 转染试剂是一种由脂质和其它成分混合而得的专利混合物，溶于80%乙醇中，经0.2 μm滤膜过滤，然后封装于玻璃小瓶中。

應用

X-treme基因™ 9 DNA转染试剂是一种非脂质体多组分试剂，用于各种实验包括细胞分析。由于它的细胞毒性极低，不太需要进行优化，即使是血清存在条件下，在许多种常用细胞系中都有很高转染效率，因此非常适合于所有的细胞分析。

X-treme基因™ 9 DNA转染试剂适合的细胞分析有：
  • 重组蛋白表达功能分析。
  • 代谢通路生理研究。
  • 使用报道基因的调节序列分析。
  • 基因表达分析。
  • 癌症研究分析。
  • 目标评估。

特點和優勢

  • 使用毒性极低，转染后细胞存活率高，生成可信任的生理学相关数据。
  • 节省时间，减少操作步骤；只需对X-tremeGENE 9 DNA转染试剂进行简单稀释，再与质粒DNA共同孵育，即可直接向细胞添加反应混合物（有无血清均可）。
  • 对于常用细胞无需进行费时费力的优化工作。

品質

每一批次的X-tremeGENE™ 9 DNA转染试剂都经过严格的质量控制流程测试，确保性能合规的标准性。在质量测试环节，首先使用X-tremeGENE™ 9 DNA转染试剂（试剂DNA比为3:1μl/μg DNA）向细胞转染一个报告基因载体。再通过化学荧光检测法监测报告基因活性。最后使用一条标准曲线对每孔的重组蛋白总量进行定量，以确定产品达到规格。

外觀

本产品溶于80%乙醇中并经0.2 μm滤膜过滤。使用次数：使用标准步骤时，在96孔板内，如使用3:1 试剂-DNA比，则1ml X-tremeGENE 9 DNA转染试剂可以进行6,600次转染；如使用2:1 试剂-DNA比，则1ml X-tremeGENE™ 9 DNA转染试剂可进行10,000转染。

其他說明

仅用于生命科学研究。不可用于诊断。

法律資訊

X-tremeGENE is a trademark of Roche

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象形圖

FlameExclamation mark
GHS02,GHS07

訊號詞

Danger

危險聲明

H225 - H319

危險分類

Eye Irrit. 2 - Flam. Liq. 2

儲存類別代碼

3 - Flammable liquids

水污染物質分類（WGK）

WGK 1

閃點（°F）

334.4 °F

閃點（°C）

168 °C

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
19129300
39320900
46261800
33315000
23644720

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Leonardo Freire-de-Lima et al.
Proceedings of the National Academy of Sciences of the United States of America, 108(43), 17690-17695 (2011-10-19)
The process termed "epithelial-mesenchymal transition" (EMT) was originally discovered in ontogenic development, and has been shown to be one of the key steps in tumor cell progression and metastasis. Recently, we showed that the expression of some glycosphingolipids (GSLs) is
L C Costantini et al.
Neuroscience, 89(2), 505-513 (1999-03-17)
To investigate the role of neurotrophins in the initial formation of striatal patch versus matrix, the spatial and temporal expression of trkB receptors was examined using immunohistochemistry. Polyclonal antibodies, against the C-terminus or the tyrosine kinase domain, revealed trkB-immunoreactive cells
Piroon Jenjaroenpun et al.
BMC genomics, 10 Suppl 3, S9-S9 (2010-01-09)
DNA triplexes can naturally occur, co-localize and interact with many other regulatory DNA elements (e.g. G-quadruplex (G4) DNA motifs), specific DNA-binding proteins (e.g. transcription factors (TFs)), and micro-RNA (miRNA) precursors. Specific genome localizations of triplex target DNA sites (TTSs) may
Lauren Rouleau et al.
Free radical biology & medicine, 95, 308-322 (2016-04-03)
We investigated the mechanism of selective ascorbate-induced cytotoxicity in tumor cells, including Hep G2 cells, compared to primary hepatocytes. H2O2 formation was required for ascorbate cytotoxicity, as extracellular catalase treatment protected tumor cells. H2O2 generated by glucose oxidase treatment also
Daniel F Comiskey et al.
Nucleic acids research, 43(8), 4202-4218 (2015-04-08)
Genotoxic stress induces alternative splicing of the oncogene MDM2 generating MDM2-ALT1, an isoform attributed with tumorigenic properties. However, the mechanisms underlying this event remain unclear. Here we explore MDM2 splicing regulation by utilizing a novel minigene that mimics endogenous MDM2
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A Versatile new Lipofection Reagent for co-transfection of DNA and RNA: X-tremeGENE™ siRNA Transfection Reagent

Small inhibitory RNAs (siRNAs) have become the focus of interest in many laboratories. For the first time, these molecules offer an easy way to knock down the expression of selected genes in mammalian cells without having to resort to classical gene knockout techniques.

Introduction to Cell Transfection

Transfection is the introduction of DNA, RNA, or proteins into eukaryotic cells and is used in research to study and modulate gene expression. Thus, transfection techniques and protocols serve as an analytical tool that facilitates the characterization of genetic functions, protein synthesis, cell growth and development.

How Transfection Works

This brief webinar provides an overview of what transfection is and the methods that are used to introduce DNA or RNA into eukaryotic cells.

实验方案

X-tremeGENE™ 9 DNA Transfection Reagent Protocol

Plate cells approx. 24 hours before transfection making sure cells are at optimal concentration (70 – 90 % confluency).

Co-transfection of Plasmid DNA

Transient co-transfection of plasmids is a method that is commonly employed for cellular protein-protein interaction studies, transcription factor studies, and gene knockdown studies using shRNA encoding plasmids.

X-tremeGENE HP DNA Transfection Reagent Protocol

Cell preparation for transfection Plate cells approx. 24 hours before transfection making sure cells are at optimal concentration (70 – 90 % confluency).

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