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生物源
Aspergillus sp. (A. oryzae)
形狀
solution
濃度
≥100000 units/mL
技術
DNA purification: suitable
適合性
suitable for nucleic acid purification
應用
cell analysis
運輸包裝
wet ice
儲存溫度
−20°C
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一般說明
来源于米曲霉的核酸酶 S1 酶具有降解由脱氧核苷酸或核糖核苷酸组成的单链寡核苷酸的能力。
應用
在一项研究中，来源于米曲霉的核酸酶 S1 被用于评估标测 DNA 突变的生物化学方法。在另一项研究中，它还被用于研究 γ-照射的大鼠脑肿瘤中的 DNA 损伤和修复。
生化／生理作用
从米曲霉分离的核酸酶 S1 具有对单链 DNA 和 RNA 磷酸二酯键的内切和外切水解活性，产生 5'-磷酸单核苷酸和 5'-磷酸寡核苷酸终产物。它用于消化 RNA 和 DNA 双链体中未退火的多核苷酸尾部和发夹环，并可用于将超螺旋 DNA 转化为线性形式。
来源于米曲霉的 SI 核酸酶能生成 DNA 断裂或脱碱基位点产生双链 DNA 断裂。
單位定義
在 37℃，pH4.6 条件下， 一单位 SI 核酸酶每分钟将 1.0 ug 的单链核酸转化成高氯酸。
外觀
含有 30mM 乙酸钠，50mM NaCl，1mM ZnCl2，50％ 甘油，2mg/ml 蛋白质的溶液
仅试剂盒组分
产品编号
说明
- 30mM Sodium acetate .25-.25 %
- 50mM Sodium chloride .29 %
- 1mM Zinc chloride .01 %
- Glycerol 50 %
- 2mg/mL Protein .2 %
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
其他客户在看
F Harada et al.
Nucleic acids research, 2(6), 865-871 (1975-06-01)
Nuclease S1 specifically hydrolizes tRNAs in their anticodon loops, forming new 5' phosphate and 3' OH ends. Some single-stranded regions are not cut by nuclease S1. The strong preference of nuclease S1 for the anticodon region can be used for
M A Chaudhry et al.
Nucleic acids research, 23(19), 3805-3809 (1995-10-11)
Defined DNA substrates containing discrete abasic sites or paired abasic sites set 1, 3, 5 and 7 bases apart on opposite strands were constructed to examine the reactivity of S1, mung bean and P1 nucleases towards abasic sites. None of
P Beard et al.
Journal of virology, 12(6), 1303-1313 (1973-12-01)
S(1) nuclease, the single-strand specific nuclease from Aspergillus oryzae can cleave both strands of circular covalently closed, superhelical simian virus 40 (SV40) DNA to generate unit length linear duplex molecules with intact single strands. But circular, covalently closed, nonsuperhelical DNA
T E Shenk et al.
Proceedings of the National Academy of Sciences of the United States of America, 72(3), 989-993 (1975-03-01)
S1 nuclease (EC 3.1.4.X), a single-strand-specific nuclease, can be used to accurately map the location of mutational alterations in simian virus 40 (SV40) DNA. Deletions of between 32 and 190 base pairs, which are at or below the limit of
S1 nuclease from Aspergillus oryzae for the detection of DNA damage and repair in the gamma-irradiated intracerebral rat gliosarcoma 9L.
P H Gutin et al.
Radiation research, 72(1), 100-106 (1977-10-01)
实验方案
Enzymatic Assay of Nuclease S1
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