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Merck

N3755

Sigma-Aldrich

微球菌核酸酶 来源于金黄色葡萄球菌

100-300 units/mg protein

别名:

微球菌核酸酶, MNase, 微球菌核酸内切酶

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About This Item

CAS号:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.54

生物源

Staphylococcus aureus

品質等級

形狀

lyophilized powder

比活性

100-300 units/mg protein

成份

Protein, ≥40% E1%/280 (Balance primarily sodium citrate)

技術

DNA purification: suitable

適合性

suitable for molecular biology

應用

diagnostic assay manufacturing

儲存溫度

−20°C

基因資訊

Staphylococcus aureus subsp. aureus Mu50 ... nuc(1120790)

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應用

来自 金黄色葡萄球菌 的核酸酶已被用于评估动物中发现的凝固酶和耐热菌株的研究中。 它也被用于研究 金黄色葡萄球菌 中编码两个热稳定核酸酶基因的表达特征。

生化/生理作用

可水解DNA的5′-磷酸二酯键。
相较于G或C侧,微球菌核酸酶可在A或T核苷酸的5′ 侧好的对DNA进行切割。

單位定義

在pH 8.8,37℃条件下,一个单元可每分钟从天然DNA产生1.0μmole的酸可溶性多核苷酸,对于混合核苷酸来说基于EM/260 = 10,000 。

其他說明

注意:1μmole单位 = 约85 A260 单位,其中A260单位相当于在pH 8.8,37℃条件下,在3.55 mL反应体积中,30分钟内ΔA260为1.0。光路= 1cm。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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分析证书(COA)

Lot/Batch Number

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Coagulase and Heat-resistant Nuclease Producing Staphylococcus epidermidis Strains from Animals
DEVRIESE, L. and P. OEDING
Journal of Applied Microbiology, 39, 197-207 (1975)
Yu Hu et al.
Foodborne pathogens and disease, 9(3), 265-271 (2012-03-03)
Thermonuclease is known as a specific virulence factor in Staphylococcus aureus. It is widely used as a genetic marker for detection of S. aureus in various types of food. Previous studies have revealed the existence of two functional thermostable nucleases
C Dingwall et al.
Nucleic acids research, 9(12), 2659-2673 (1981-06-25)
The substrate specificity of micrococcal nuclease (EC 3.1.4.7.) has been studied. The enzyme recognises features of nucleotide composition, nucleotide sequence and tertiary structure of DNA. Kinetic analysis indicates that the rate of cleavage is 30 times greater at the 5'
Frank J Hernandez et al.
Nature medicine, 20(3), 301-306 (2014-02-04)
Technologies that enable the rapid detection and localization of bacterial infections in living animals could address an unmet need for infectious disease diagnostics. We describe a molecular imaging approach for the specific, noninvasive detection of S. aureus based on the
Joana Segura et al.
Nature communications, 9(1), 3989-3989 (2018-09-30)
The interplay between chromatin structure and DNA topology is a fundamental, yet elusive, regulator of genome activities. A paradigmatic case is the "linking number paradox" of nucleosomal DNA, which refers to the incongruence between the near two left-handed superhelical turns

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