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10107921001

Roche

核酸酶S7

Micrococcal nuclease, from Staphylococcus aureus

别名:

s7核酸酶

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About This Item

分類程式碼代碼:
12352204

生物源

Staphylococcus aureus

品質等級

100

形狀

lyophilized

比活性

~15,000 U/mg

包裝

pkg of 15,000 U

製造商／商標名

Roche

最適pH

7.5

運輸包裝

wet ice

相关类别

應用

  • 从体外翻译系统中去除内源性RNA
  • RNA测序实验
核酸酶S7已用于核糖体分析的核糖核酸酶选择过程研究。

生化／生理作用

核酸酶S7是一种核酸内切酶，主要切割单链底物。它还切割双链DNA或RNA。它切割RNA和DNA的5′磷酸二酯键，产生3′-单核苷酸和寡核苷酸。酶的活性取决于Ca2+ ，添加EDTA可使酶完全灭活。相比于DNA上的GC，核酸酶S7切割对富含AT的区域高度特异。

單位定義

一单位酶释放的足够量的酸溶性寡核苷酸可使A260增加1.0。

儲存和穩定性

未开封试剂储存于2-8 °C；1 mg/mL的稀释液则储存于-15 to -25 °C。

其他說明

仅用于生命科学研究。不可用于诊断。

象形圖

Exclamation markHealth hazard
GHS07,GHS08

訊號詞

Danger

危險分類

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3

標靶器官

Respiratory system

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 1

閃點（°F）

does not flash

閃點（°C）

does not flash

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Reimo Zoschke et al.
Proceedings of the National Academy of Sciences of the United States of America, 112(13), E1678-E1687 (2015-03-17)
Chloroplast genomes encode ∼ 37 proteins that integrate into the thylakoid membrane. The mechanisms that target these proteins to the membrane are largely unexplored. We used ribosome profiling to provide a comprehensive, high-resolution map of ribosome positions on chloroplast mRNAs
Serap Erkek et al.
Nature structural & molecular biology, 20(7), 868-875 (2013-06-19)
In mammalian spermatozoa, most but not all of the genome is densely packaged by protamines. Here we reveal the molecular logic underlying the retention of nucleosomes in mouse spermatozoa, which contain only 1% residual histones. We observe high enrichment throughout
Ribonuclease selection for ribosome profiling.
Gerashchenko M V and Gladyshev V N
Nucleic Acids Research, 45(2), e6-e6 (2016)
Maxim V Gerashchenko et al.
Nucleic acids research, 49(2), e9-e9 (2020-12-03)
There has been a surge of interest towards targeting protein synthesis to treat diseases and extend lifespan. Despite the progress, few options are available to assess translation in live animals, as their complexity limits the repertoire of experimental tools to
Sarah O'Keefe et al.
bioRxiv : the preprint server for biology (2020-12-04)
In order to produce proteins essential for their propagation, many pathogenic human viruses, including SARS-CoV-2 the causative agent of COVID-19 respiratory disease, commandeer host biosynthetic machineries and mechanisms. Three major structural proteins, the spike, envelope and membrane proteins, are amongst
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