推荐产品
一般說明
DNase I是一种可降解DNA的双链特异性内切核酸酶。牛胰腺脱氧核糖核酸酶I（DNase I）是DNA小沟相互作用的核酸酶，特异性相对较低。该蛋白质由两个中心β折叠构成，而每个折叠由六个β-链所组成。该结构被大量的环以及α-螺旋区域包围。该酶与核酸外切酶III具有结构相似性。DNase I是研究得最透彻的哺乳动物来源的内切酶之一。
應用
DNase I 与胶原酶一起已被用于组织解离。
生化／生理作用
在组织解离过程中，部分细胞被裂解并导致DNA的释放。单分子DNA可能会导致细胞结团。向解离缓冲液中加入DNase I会导致该细胞外DNA的降解，因而避免了因意外结团造成的细胞损失。
特點和優勢
- 冻干的
- 且冻干前经 0.2 μm 孔径膜过滤
- 糖蛋白和双链特异性内切酶
單位定義
一个单位表示在测定条件下导致吸光度以0.001每分钟的速率而增加的酶活力。
準備報告
激活剂：二价金属离子 (Ca2+, Mg2+)
工作浓度：0.01 至 1 mg/ml
注意：必须针对每种细胞类型单独确定工作浓度。为实现最佳活性，酶须添加 5 mM Mg2+。
工作浓度：0.01 至 1 mg/ml
注意：必须针对每种细胞类型单独确定工作浓度。为实现最佳活性，酶须添加 5 mM Mg2+。
重構
用无菌的双蒸水对冻干酶进行重悬（10 mg/ml）；用PBS（磷酸盐缓冲液）、HBSS（Hank′s 平衡盐溶液）或培养基进行进一步的稀释。
其他說明
仅用于生命科学研究。不用于诊断操作。
訊號詞
Danger
危險聲明
危險分類
Resp. Sens. 1 - Skin Sens. 1
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 1
閃點（°F）
does not flash
閃點（°C）
does not flash
其他客户在看
Karoline Möller et al.
Experimental & translational stroke medicine, 4(1), 20-20 (2012-10-04)
The pathophysiology of sterile inflammation following focal ischemic stroke is complex and not fully understood, but there is growing evidence that it offers several therapeutic options beyond the hitherto existing treatment strategies. The identification and quantification of infiltrating inflammatory cells
Burkhard Steuernagel et al.
Plant physiology, 183(2), 468-482 (2020-03-19)
Disease resistance genes encoding nucleotide-binding and leucine-rich repeat (NLR) intracellular immune receptor proteins detect pathogens by the presence of pathogen effectors. Plant genomes typically contain hundreds of NLR-encoding genes. The availability of the hexaploid wheat (Triticum aestivum) cultivar Chinese Spring
Gareth B Kitchen et al.
Proceedings of the National Academy of Sciences of the United States of America, 117(3), 1543-1551 (2020-01-05)
The circadian clock regulates many aspects of immunity. Bacterial infections are affected by time of day, but the mechanisms involved remain undefined. Here we show that loss of the core clock protein BMAL1 in macrophages confers protection against pneumococcal pneumonia.
Yutaka Takeuchi et al.
Biology of reproduction, 81(6), 1055-1063 (2009-07-17)
In a recently established system for intraperitoneal spermatogonial cell transplantation in salmonids, donor type A spermatogonia (type A SG) were microinjected into the peritoneal cavity of newly hatched larvae. Compared with salmonids, the larvae of marine teleosts are small and
Fallen Teoh et al.
Environmental microbiology, 22(5), 1816-1828 (2019-11-27)
Marine cyanobacteria genus Synechococcus are among the most abundant and widespread primary producers in the open ocean. Synechococcus strains belonging to different clades have adapted distinct strategies for growth and survival across a range of marine conditions. Clades I and
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门