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H4272

Sigma-Aldrich

透明质酸酶 来源于牛睾丸

Type IV-S, powder, suitable for mouse embryo cell culture, 750-3000 units/mg solid

别名:

玻璃糖醛酸酶, 玻璃酸酶

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About This Item

CAS号:
37326-33-3
EC 号:
3.2.1.35 (BRENDA, IUBMB)
EC號碼:
253-464-3
MDL號碼:
MFCD00131351
分類程式碼代碼:
12352204
NACRES:
NA.75

無菌

aseptically processed

品質等級

200

種類

Type IV-S

形狀

powder

比活性

750-3000 units/mg solid

分子量

~55 kDa (four subunits of 14 kDa each)

成份

Protein, 80-105% biuret

技術

cell culture | embryo: suitable

儲存溫度

−20°C

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相关类别

應用

透明质酸酶是一种来自牛睾丸的酶。准备用于体外培养的卵子时，可利用这种酶去除小鼠胚胎分离物或新排卵中的卵丘物质。

促性腺激素用于使20只雌性小鼠超排卵。然后小鼠进行交配，获得并计数着床前期胚胎。然后胚胎使用透明质酸酶处理，知道其中所有积云物质都从胚胎里去除掉。胚胎被培育，每天查看，记录胚胎发育进展。

生化／生理作用

这些酶可随机裂解透明质酸、软骨素和硫酸软骨素中的 β-N-乙酰己糖胺-[1→4] 糖苷键。
透明质酸酶可降解透明质酸，并已发现可在癌症进展期间会被不适当地调节。

單位定義

在 37 ºC、pH 5.35 条件下，一单位每分钟可降解 0.75 μg 多糖透明质酸（通过比浊吸光度测量(λ: 600nm)，与 BSA 复合 45 分钟后测量）。

準備報告

用3 ml M2培养基（货号M 7167或M 5910）或去离子水复溶1瓶透明质酸酶，获得10 mg/ml的储备溶液。用M2培养基稀释储备溶液，至约300 μg/ml最终工作浓度。

象形圖

Health hazard
GHS08

訊號詞

Danger

危險聲明

H334

防範說明

P261 - P284 - P501

危險分類

Resp. Sens. 1

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)

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Liesl Jacobs et al.
Molecular therapy : the journal of the American Society of Gene Therapy, 28(4), 1068-1077 (2020-02-27)
Checkpoint-inhibiting antibodies elicit impressive clinical responses, but still face several issues. The current study evaluated whether DNA-based delivery can broaden the application of checkpoint inhibitors, specifically by pursuing cost-efficient in vivo production, facilitating combination therapies, and exploring administration routes that lower
Yu Shi et al.
Nature communications, 8(1), 2043-2043 (2017-12-13)
Bone formation in mammals requires continuous production of osteoblasts throughout life. A common molecular marker for all osteogenic mesenchymal progenitors has not been identified. Here, by lineage-tracing experiments in fetal or postnatal mice, we discover that Gli1
Wan Kin Au Yeung et al.
Cell reports, 27(1), 282-293 (2019-04-04)
Mammalian histone methyltransferase G9a (also called EHMT2) deposits H3K9me2 on chromatin and is essential for postimplantation development. However, its role in oogenesis and preimplantation development remains poorly understood. We show that H3K9me2-enriched chromatin domains in mouse oocytes are generally depleted
Marco Ragusa et al.
RNA biology, 16(9), 1237-1248 (2019-05-29)
Circular RNAs (circRNAs) have a critical role in the control of gene expression. Their function in spermatozoa (SPZ) is unknown to date. Twenty-eight genes, involved in SPZ/testicular and epididymal physiology, were given in circBase database to find which of them
P Lorenzo Bozzelli et al.
Experimental neurology, 323, 113077-113077 (2019-11-05)
HIV-associated neurocognitive disorders (HAND) continue to persist despite effective control of viral replication. Although the mechanisms underlying HAND are poorly understood, recent attention has focused on altered neuronal population activity as a correlate of impaired cognition. However, while alterations in
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