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一般說明
大肠杆菌B株是k-12后研究最广泛的DNA。大肠杆菌B株和k-12的基因在IS序列元素上存在差异。菌株B具有B岛,表达o特异性侧链LPS。这是未剪接的基因组DNA。
特異性
50% GC;Tm = 90.5 °C
應用
DNA特别适用于基因组分析,包括PCR、噬菌体λ的文库构建以及异源性预杂交和杂交方案。
来自大肠杆菌菌株B的脱氧核糖核酸钠盐已被用于:
来自大肠杆菌菌株B的脱氧核糖核酸钠盐已被用于:
- 阴道加德菌基因组DNA的PCR反应
- dNTP的缺口翻译和放射性标记
- 生成用于16S rDNA定量的标准曲线
- 转染中性粒细胞
- 测试提取方法
單位定義
一个酶活性单位是指在pH 7.5(1 cm光程)条件下,在1.0 ml的1 mM Tris-HCl溶液(含有1 mM NaCl和1 mM EDTA)中产生1.0的 A260 所需的酶量。
準備報告
通过阴离子交换色谱法纯化,使用含有1 mM氯化钠、1 mM EDTA和1 mM pH 7.5的 Trizma Hcl析出并以大约2单位/L的浓度冷冻干燥。每mg DNA大约20 A260个单位。 %GC是G/C碱基对的百分比。Tm是热变性曲线中点处的温度。
分析報告
在溴化乙锭染色后通过凝胶电泳和可视化分析,具有45kB或更大的单一主条带。
其他說明
溶液中的DNA在室温下放置后将退火,因此,建议将溶液煮沸10分钟,然后在使用前在冰上冷却至少5分钟。
相關產品
产品编号
说明
价格
儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 3
閃點(°F)
Not applicable
閃點(°C)
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
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