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一般說明
从雄性和雌性小牛的胸腺分离的高质量双链模板DNA。
特異性
42% GC;Tm = 87 °C.
%GC是指G/C碱基对的百分比。Tm是热变性曲线中点处的温度。
%GC是指G/C碱基对的百分比。Tm是热变性曲线中点处的温度。
應用
小牛胸腺DNA非常适合用作DNA聚合酶检测的底物、用于扩增非常长的片段以及用作DNA沉淀的载体。它被用作DNA荧光定量的标准品。它被用作参考物质,用于检测软骨组织消化样品的DNA含量。
小牛胸腺DNA非常适合用于作为DNA聚合酶测定的底物、长片段的扩增、以及作为沉淀时的载体DNA。
特點和優勢
•在37°C温育16小时后未检测到核酸酶活性
•高质量的模板DNA
•高质量的模板DNA
準備報告
阴离子交换色谱纯化,用含1mM氯化钠、1mM EDTA和1mM Trizma HCl的溶液(pH 7.5)透析,并以约2 A260 单位/mL的浓度冻干。约20 260单位/mg DNA。
重構
将DNA溶解在分子生物级水中并轻轻摇动过夜,使终浓度为1mg/ml。
儲存類別代碼
10 - Combustible liquids
水污染物質分類(WGK)
WGK 3
閃點(°F)
Not applicable
閃點(°C)
Not applicable
個人防護裝備
Eyeshields, Gloves
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