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Merck

D4522

Sigma-Aldrich

脱氧核糖核酸 来源于小牛胸腺

Type XV, Activated, lyophilized powder

别名:

DNA 来源于小牛胸腺, 小牛胸腺DNA, 胸腺核酸

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About This Item

CAS号:
EC號碼:
MDL號碼:
分類程式碼代碼:
41106310
eCl@ss:
32160414
NACRES:
NA.52

等級

for molecular biology

品質等級

種類

Type XV

形狀

lyophilized powder

儲存溫度

−20°C

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一般說明

从雄性和雌性小牛的胸腺分离的高质量双链模板DNA。

應用

小牛胸腺DNA非常适合用于作为DNA聚合酶测定的底物、长片段的扩增、以及作为沉淀时的载体DNA。它被用作确定凝胶中DNA含量的标准品 以及通过荧光检测对DNA进行定量。
小牛胸腺DNA非常适合用于作为DNA聚合酶测定的底物、长片段的扩增、以及作为沉淀时的载体DNA。

特點和優勢

•在37°C温育16小时后未检测到核酸酶活性
•高质量的模板DNA

數量

1mg DNA约为20个 A260 单位。

準備報告

使用Aposhian和Kornberg的方法,从高度聚合的小牛胸腺DNA中进行制备,并用DNase进行切口。

重構

将DNA在分子生物级水中溶解过夜,终浓度为1mg/ml。

相關產品

产品编号
说明
价格

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves


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Claudia Gomes et al.
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High levels of autoimmune antibodies are observed in COVID-19 patients but their specific contribution to disease severity and clinical manifestations remains poorly understood. We performed a retrospective study of 115 COVID-19 hospitalized patients with different degrees of severity to analyze
Parul Natvar Patel et al.
Tissue engineering, 11(9-10), 1498-1505 (2005-11-02)
The ultimate goal of this research is to develop an injectable cell-scaffold system capable of permitting adipogenesis to abrogate soft tissue deficiencies resulting from trauma, tumor resection, and congenital abnormalities. The present work compares the efficacy of photopolymerizable poly(ethylene glycol)
Anastasia S Singatulina et al.
Cell reports, 27(6), 1809-1821 (2019-05-09)
PARP-1 synthesizes long poly(ADP-ribose) chains (PAR) at DNA damage sites to recruit DNA repair factors. Among proteins relocated on damaged DNA, the RNA-binding protein FUS is one of the most abundant, raising the issue about its involvement in DNA repair.
Enzymatic synthesis of deoxyribonucleic acid. IX. The polymerase formed after T2 bacteriophage infection of Escherichia coli: a new enzyme.
H V APOSHIAN et al.
The Journal of biological chemistry, 237, 519-525 (1962-02-01)
Benjamin D Dickson et al.
Molecules (Basel, Switzerland), 24(8) (2019-04-24)
Poly(ADP-ribose)polymerase (PARP) inhibitors (PARPi) have recently been approved for the treatment of breast and ovarian tumors with defects in homologous recombination repair (HRR). Although it has been demonstrated that PARPi also sensitize HRR competent tumors to cytotoxic chemotherapies or radiotherapy

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