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DNAQF

Sigma-Aldrich

DNA 定量试剂盒（荧光法）

Quantitation of DNA using bisBenzimide

别名:

dsDNA quantification

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About This Item

分類程式碼代碼:
41105500
NACRES:
NA.84

品質等級

200

用途

sufficient for 750 assays (2 ml each)

技術

nucleic acid detection: suitable

儲存溫度

−20°C

一般說明

该试剂盒使用荧光染料bisBenzimide（Hoechst 33258）对DNA进行定量所需的所有试剂。该试剂盒可以高灵敏、快速、准确地测定10ng/ml至5μg/ml的双链DNA，即使存在RNA和蛋白质的情况下也是如此。荧光测定法是一种灵敏度高、简单易行的DNA定量方法。荧光染料bisBenzimide（Hoechst 33258）主要结合双链DNA（dsDNA）小沟内的AT序列，专门用于量化DNA的毫微克量（10 ng/ml至10 mg/ml）。当在360 nm激发时，DNA环境下染料在460 nm处的荧光发射显著增加。

應用

DNA定量试剂盒，荧光法已用于测量DNA浓度/含量：
  • 新生大鼠心室肌细胞(NRVM)
  • 在人间充质干细胞(hMSC)/样品中，进行相对碱性磷酸酶(ALP)量标准化
  • 小鼠胚胎成纤维细胞（NIH3T3细胞）

特點和優勢

  • 专用于ng级别DNA的定量
  • 适用于纯化制备物，和纯化含有RNA和蛋白质的粗提取物中的DNA

仅试剂盒组分

产品编号
说明
  • Detailed technical bulletin 1 ea

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儲存類別代碼

10 - Combustible liquids

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