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生物源
eel liver
增長模式
Adherent
染色體組型
Not specified
形態學
Fibroblast-like
產品
Not specified
受體
Not specified
技術
cell culture | mammalian: suitable
運輸包裝
dry ice
儲存溫度
−196°C
細胞系來源
Eel liver
細胞系描述
The hepatocyte cell line Hepa-E1 was derived from water eel. The cells show no contact inhibition and have been reported to show apoptosis in the presence of actinomycin D, humic acid or TGF-β 1. Normal culture conditions are at 26-28°C, but maintenance at 4°C for up to 400 days was described as well.
培養基
E-RDF (Kyokuto Pharmaceuticals)+ 2mM Glutamine + 5% Foetal Bovine Serum (FBS).
例行更新培養
Split sub-confluent cultures (70-80%) 1:4 to 1:8 using 0.25% trypsin or trypsin/EDTA 26-28°C.
其他說明
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