NCTC clone 1469

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 88111403, mouse liver, Epithelial

• 分類程式碼代碼: 41106514

属性

• 生物源: mouse liver
• 包裝: tube of 5 μg 88111403-DNA-5UG; pkg of vial of cells 88111403-1VL
• 增長模式: Adherent
• 染色體組型: Not specified
• 形態學: Epithelial
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Mouse C3H/An liver

細胞系描述

Derived from mouse liver cell line NCTC 721 which was obtained from normal liver of a 2 day old C3H/An mouse. The cells have been used in biochemical and nutritional studies. The cells are susceptible to VSV (Indiana strain).

應用

Cell suspension systems. Biochemical, nutritional and virus studies. VSV(Indiana)

培養基

NCTC 135 + 2mM Glutamine + 10% Horse Serum (HS)

例行更新培養

Split sub-confluent cultures (70-80%) 1:2 to 1:4 using only mechanical techniques, such as scraper, do not use trypsin, 5-10CO₂; 37°C. Change media every 3 days. Freeze in 10% glycerol/90% FBS. Cells are shed into the medium giving it a turbid appearan

其他說明

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