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生物源
mouse liver
增長模式
Adherent
染色體組型
Not specified
形態學
Not specified
產品
Albumin, alpha-foetoprotein, alpha 1-antitrypsin, amylase
受體
Not specified
技術
cell culture | mammalian: suitable
相關疾病
cancer
運輸包裝
dry ice
儲存溫度
−196°C
細胞系來源
Mouse hepatoma
細胞系描述
Derived from BW7756 tumour in a C57L mouse. Secretes several liver products including albumin, α-fetoprotein, α 1-antitrypin and amylase.
應用
Hepa 1-6 has been used to study the biodistribution of polymer hydrogel capsules for the delivery of therapeutics. It has also been used to evaluate the potential of interferon regulatory factor-1 (IRF-1) for cytokine gene therapy of hepatocellular carcinoma (HCC).
培養基
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
例行更新培養
Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-5x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
其他說明
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