95102433

BEAS-2B Cell Line human

• 分類程式碼代碼: 41106514

属性

• 生物源: human lung
• 品質等級: 100
• 描述: Human bronchial epithelium, normal
• 形狀: liquid
• 增長模式: Adherent
• 染色體組型: Not specified
• 形態學: Epithelial-like
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Human bronchial epithelium, normal

細胞系描述

BEAS-2B cells were derived from normal bronchial epithelium obtained from autopsy of non-cancerous individuals. Cells were infected with a replication-defective SV40/adenovirus 12 hybrid and cloned. Squamous differentiation can be observed in response to serum. This ability can be used for screening chemical and biological agents inducing or affecting differentiation and/or carcinogenesis. The cell line has been applied for studies of pneumococcal infection mechanisms. BEAS-2B was described to express keratins and SV40 T antigen. Subculturing the cells before confluency is necessary as confluent cultures rapidly undergo squamous terminal differentiation.

應用

BEAS-2B Cell line has been used to study differentiation of squamous cells and effect of biological and chemical agents on differentiation.

DNA分析

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 9,12
D13S317: 13
D16S539: 12
D5S818: 12,13
D7S820: 10,13
THO1: 7,9.3
TPOX: 6,11
vWA: 17,18

培養基

BEGM, also known as LHC-9 with modification (available from Clonetics Corporation, CC3171 (BEBM) plus additives CC4175 or BEGM Bullet Kit, CC3170); Note: Additives supplied contain gentamycin which has been omitted for culture at ECACC; media is serum-free.

例行更新培養

Passage sub-confluent cultures using 0.25% trypsin or trypsin/EDTA. Incubate at room temperature for 5-10 min until cells detach. Add fresh medium and disperse cells, centrifuge and resuspend pellet in medium. Seed into new flasks at 1500 to 3000 cells per cm². Flasks have to be precoated with a mixture of 0.01 mg/ml fibronectin, 0.03 mg/ml collagen and 0.001 mg/ml bovine serum albumin dissolved in BEGM. Add mixture at ratio of 0.2 ml per cm² surface area. Incubate at 5% CO₂, 37°C for at least 6 hours at 37oC. Remove excess fluid and allow flasks to dry by incubating at 37oC overnight, leaving the caps loose. Prior to addition of cells wash flask three times with PBS. Note: BEGM medium is almost serum-free, therefore trypsin inhibitor is essential. Add an equal or greater volume of trypsin inhibitor as trypsin used before, centrifuge and reseed cells in collagen coated flasks using culture medium.

其他說明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line.
Please view the Terms & Conditions of Supply for more information.

免責聲明

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

安全信息

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable