PC-9 (formerly known as PC-14)

90071810, human lung, Heterogeneous mixture of round cells and spindle shaped cells

• 别名: PC-9/S1 Cells, PC-9S1 Cells, PC9 Cells
• 分類程式碼代碼: 41106514

属性

• product name: PC-9 (formerly known as PC-14), 90071810
• 生物源: human lung
• 增長模式: Adherent/suspension
• 染色體組型: Not specified
• 形態學: Heterogeneous mixture of round cells and spindle shaped cells
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Human adenocarcinoma

細胞系描述

Derived from a human adenocarcinoma from lung tissue which remains undifferentiated.

PC-14 was originally deposited with the Riken BioResource Center as a cell line derived from human lung adenocarcinoma (undifferentiated type) in 1989. The RIKEN BioResource Center subsequently informed the European Collection of Cell Cultures (ECACC) that short tandem repeat (STR) DNA profile analysis, undertaken in collaboration Immuno-Biological Laboratories Co., Ltd Japan, has shown this cell line to be identical to PC-9 a cell line derived from human lung adenocarcinoma (differentiated type). The misidentification occurred prior to the cell line being deposited at the RIKEN BioResource Center. The name of the cell line has been changed to PC-9 to reflect this finding.

應用

PC-9 cell line has been used to study the effects of α-tocopherol addition on various cytotoxic drugs.

Tumourigenicity studies

DNA分析

STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 8
D16S539: 9
D5S818: 11
D7S820: 10
THO1: 7
TPOX: 11
vWA: 17

培養基

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

例行更新培養

Seed cells at 2-4 x 10,000 cells/cm²; 5% CO₂; 37°C. Remove attached cells by brief treatment with 0.25% trypsin or trypsin/EDTA.  

其他說明

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