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Merck

AMPD1

Sigma-Aldrich

脱氧核糖核酸酶I

Amplification Grade

别名:

脱氧核糖核酸酶 I(DNA酶I)

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About This Item

分類程式碼代碼:
12352200
NACRES:
NA.55

品質等級

形狀

liquid

濃度

1 unit/μL

技術

RT-PCR: suitable

顏色

colorless

運輸包裝

wet ice

儲存溫度

−20°C

一般說明

脱氧核糖核酸酶I(DNase I)是一种从牛胰腺中分离的内切核酸酶,可将双链和单链DNA消化成寡核苷酸和单核苷酸。 扩增级 DNase I 已纯化去除RNase活性,适于在 RT-PCR(逆转录酶 - 聚合酶链反应)等敏感应用之前去除 RNA 制剂中的DNA。

DNase I可将双链和单链DNA酶解成寡核苷酸和单核苷酸。采用提供的反应缓冲液,在室温下消化 15 分钟,可去除 RNA 制剂中的 DNA。然后随同终止液加热,使DNase I失活。加热还会使RNA中的发夹变性,可直接用于逆转录。

当前没有任何 RNA 分离程序可以 100% 去除 DNA。 许多商业DNase I 制剂受到了残留RNase污染。 这种RNase污染会在逆转录以前破坏或分解有价值的RNA样品。实验室比较表明,Sigma′的扩增级 DNase I 的RNase活性低于几家领先的分子生物学产品供应商产品。

應用

扩增级脱氧核糖核酸酶I(DNase I)已用于:
  • 在 RNA 的分离和纯化过程中消化 DNA。纯化的 RNA 可用于通过RNA 逆转录酶合成 cDNA。
  • 水解细胞外基质 (ECM) 成分并增强光敏剂在生物膜中的渗透以确定抗菌光动力疗法 (aPDT) 对白色念珠菌生物膜的功效
  • 从牛血样中提取的总 RNA 中去除污染 DNA

特點和優勢

  • 适用于去除 RNA 中的 DNA
  • 超低RNase活性
  • 经过优化的10×反应缓冲液和终止液,可使DNase I完全失活

適合性

适用于去除 RNA 制剂中的 DNA。

單位定義

在 37 °C 下,一个单位可在 10 min 内将 1μg 质粒DNA 消化为寡核苷酸。

法律資訊

购买本产品附带有限的许可,仅可用于研究用途聚合酶链式反应 (PCR) 过程,且应配合热循环仪使用。该热循环仪在 PCR 过程的自动执行中的使用由预先许可费涵盖,该费用通过向 Applied Biosystems 支付或购买的方式(即授权的热循环仪)支付。

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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访问文档库

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实验方案

The SeqPlex RNA Amplification kit provides a method for amplification of total RNA or isolated mRNA prior to entry into the workflows of the commonly used deep sequencing platforms.

Method for reverse transcription of RNA into DNA. Uses a premixed reagent that contains reverse transcriptase, dNTPs, primers, RNase inhibitor and buffer. Fast generation of cDNA.

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Polymerase chain reaction (PCR) is a technique for amplifying nucleic acid molecules and is commonly used in many applications, including RT-PCR, hot start PCR, end point PCR and more.

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