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一般說明
eAMV™ 逆转录酶是一种增强型禽成髓细胞病病毒（AMV）逆转录酶，负责合成与 RNA、DNA 或 RNA:DNA 杂交体互补的 DNA 链。这种异常强大的 AMV逆转录酶比标准 AMV 或 Moloney 鼠白血病病毒（M-MLV）逆转录酶具有更优的热稳定性。eAMV™ RT 是从总 RNA 或 poly（A）+RNA获得高质量全长 cDNA 的理想酶，并且在转录长靶标方面也是有效的。
應用
Enhanced Avian Reverse Transcriptase可将RNA转录为DNA，同时显著提升mRNA模板合成cDNA的效率。这是由于本款增强型AMV-RT能够逆转录长链mRNA模板，能够有效穿越复杂的二级结构，可以通过RT-PCR反应检测低丰度mRNA。
增强型禽逆转录酶[eAMV™逆转录酶]已用于总 RNA 的逆转录，以在定量逆转录 PCR（RT-qPCR）分析中合成 cDNA。
生化／生理作用
逆转录酶能够加速dNTP形成磷酸二酯键，从而催化RNA模板整合到互补DNA中。
特點和優勢
- 对低丰度信使核糖核酸的敏感性更高
- 在高温（高达 65°C）下通过复杂二级结构进行的无核转录
- 高效生成高达 14.1kb的全长 cDNA
- 产生可用于 PCR 扩增的第一链 cDNA
包裝
提供1管10x反应缓冲液。
單位定義
一单位酶能够以多聚腺苷酸为模板，以12-18的oligo(dT)为引物，在10分钟内将1纳摩TMP整合进TCA沉淀材料中。
法律資訊
Purchase of this product is accompanied by a limited license for use in the Polymerase Chain Reaction (PCR) process for research purposes only and in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by an up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., and authorized thermal cycler.
eAMV is a trademark of Sigma-Aldrich Co. LLC
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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One approach to the analysis of gene expression is to measure the concentration of mRNA of a gene. There are several challenges to such analyses, such as the differences in half life between different transcripts, the temporal patterns of transcription and the lack of correlation between mRNA and protein.
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