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Merck
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主要文件

71110-M

Millipore

rLysozyme溶液

Stabilized recombinant lysozyme

别名:

recombinant lysozyme

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About This Item

分類程式碼代碼:
12352202
NACRES:
NA.77

重組細胞

expressed in E. coli

品質等級

400

形狀

solution

製造商／商標名

Novagen®

儲存條件

OK to freeze
avoid repeated freeze/thaw cycles

儲存溫度

−20°C

一般說明

rLysozyme溶液含有高度纯化和稳定的重组溶菌酶，可用于裂解革兰氏阴性细菌，例如大肠杆菌。该酶催化细菌细胞壁中N-乙酰胞壁质键的水解。rLysozyme（1700 kU/mg）对大肠杆菌裂解的比活性比鸡卵清溶菌酶大250倍。rLysozyme在生理pH下具有最佳活性。非常少量的rLysozyme（3–5 kU/g细胞糊）可提高BugBuster、BugBuster HT和PopCulture试剂的蛋白质提取效率。在没有蛋白质提取试剂的情况下，用45–60 KU rLysozyme处理1.0 g细胞糊，可以直接裂解大肠杆菌。该产品以即用型溶液的形式提供，浓度范围为27–33 KU/µl，在50%甘油中含有50 mM Tris-HCl、0.1 M NaCl、0.1 mM EDTA、1 mM DTT、0.1% TRITON X-100，pH 7.5。rLysozyme溶液在–20°C保持稳定。

警告

毒性：标准处理（A）

單位定義

一单位rLysozyme定义为在0.5 x BugBuster中用50mM Tris-HCl，pH 7.5稀释的1 ml Tuner(DE3)细胞悬液（1 mg/ml）在25°C下每分钟降低0.025 A₄₅₀单位所需的酶量。

法律資訊

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

儲存類別代碼

10 - Combustible liquids

水污染物質分類（WGK）

WGK 2

分析证书(COA)

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