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生物源
chicken egg white
品質等級
形狀
liquid
分子量
single-chain 14.3 kDa
濃度
≥400000 units/mL
10 mg/mL
技術
cell based assay: suitable
適合性
suitable for cell lysis
UniProt登錄號
應用
cell analysis
運輸包裝
wet ice
儲存溫度
−20°C
基因資訊
chicken ... LYZ(396218)
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一般說明
溶酶体是一种存在于蛋清中的酶，它能分解细菌细胞壁，提高提取 DNA 和蛋白质的效率。
應用
适用于细菌 DNA 和蛋白质的纯化。
酶可分解细菌的细胞壁；用于制备原生质体。
生化／生理作用
溶菌酶可水解肽聚糖中N-乙酰胞壁酸与N-乙酰-D-氨基葡萄糖残基之间以及壳糊精中N-乙酰-D-氨基葡萄糖残基之间的β（1→4）连接。 革兰氏阳性细胞对这种水解非常敏感，因为它们的细胞壁中的肽聚糖比例很高。革兰氏阴性菌就对这种水解没那么敏感，因为它们有外膜并且肽聚糖的比例较低。然而，这些细胞可能在细胞外膜中螯合金属离子的EDTA存在的情况下水解。
该酶在广泛的pH范围（6.0至9.0）内具有活性。pH值为6.2时，可在更广的离子强度范围（0.02至0.100 M）观察到最大活性，而pH值为9.2时为0.01至0.06 M。
该酶在广泛的pH范围（6.0至9.0）内具有活性。pH值为6.2时，可在更广的离子强度范围（0.02至0.100 M）观察到最大活性，而pH值为9.2时为0.01至0.06 M。
適合性
采用 CelLytic ™ B 从细菌中提取蛋白质的适用性试验。
外觀
50% 甘油、25 mM 醋酸钠溶液
即用型溶液：10 mg/mL溶菌酶，溶于含50%甘油的25 mM醋酸钠（pH 4.5）中。
法律資訊
CelLytic is a trademark of Sigma-Aldrich Co. LLC
訊號詞
Danger
危險聲明
危險分類
Resp. Sens. 1
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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