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Merck

P4978

Sigma-Aldrich

Phosphatase, Alkaline from calf intestine

buffered aqueous glycerol solution

Sinónimos:

CIAP, CIP, Orthophosphoric-monoester phosphohydrolase (alkaline optimum)

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About This Item

Número de CAS:
Comisión internacional de enzimas:
Número MDL:
Código UNSPSC:
12352204
eCl@ss:
42010105
NACRES:
NA.53

grado

for molecular biology

formulario

buffered aqueous glycerol solution

mol peso

~80 kDa

concentración

≥10,000 units/mL

Nº de acceso UniProt

actividad extraña

DNase, RNase, none detected

Condiciones de envío

wet ice

temp. de almacenamiento

−20°C

Información sobre el gen

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Descripción general

Alkaline phosphatase is a phosphomonoesterase that catalyzes the cleavage of terminal phosphates from nucleic acids. The free 5′-OH can be phosphorylated with polynucleotide kinase and γ32P-ATP to produce γ32P-end-labeled nucleic acids. Linearized cloning vectors can be prevented from recircularizing by dephosphorylation with alkaline phosphatase.

Aplicación

Commonly used to remove the 5′-terminal phosphate from nucleic acids during molecular cloning reactions.

Componentes

Alkaline phosphatase is provided in a solution of 10 mM Tris-HCl (pH 8.2), 50 mM KCl, 1 mM MgCl2, and 0.1 mM ZnCl2, in 50% (w/v) glycerol. 10X CIP Buffer, Product Number C3225, is included.

Definición de unidad

One unit will hydrolyze 1 μmole of p-nitrophenyl phosphate per min at 37 °C.

Otras notas

Alkaline phosphatase in 5 mM EDTA (pH 8) will be irreversibly heat inactivated at 75 °C for 10 minutes.

sustrato

Referencia del producto
Descripción
Precios

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


Certificados de análisis (COA)

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Protocolos

CIP is used to remove 5’-phosphate groups from DNA, RNA and both ribo and deoxy-ribonucleoside triphosphates. Detailed protocol on how to dephosphorylate DNA.

Enzymatic Assay of Alkaline Phosphatase, Diethanolamine Assay (EC 3. 1. 3. 1)

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