D7291

Deoxyribonuclease I RNase-free solution from bovine pancreas

• Sinónimos: DNase I, Deoxyribonuclease I, pancreatic DNase I, pancreatic deoxyribonuclease I
• Número de CAS: 9003-98-9
• Comisión internacional de enzimas: 3.1.21.1 (BRENDA, IUBMB)
• Número CE: 232-667-0
• Número MDL: MFCD00130918
• Código UNSPSC: 12352204
• NACRES: NA.53

Propiedades

• origen biológico: bovine pancreas
• grado: for molecular biology
• Formulario: buffered aqueous glycerol solution
• actividad específica: 10000 units/mg protein
• mol peso: 29.1 kDa
• concentración: 5—15 mg protein/mL
• técnicas: DNA extraction: suitable
• idoneidad: suitable for molecular biology
• Nº de acceso UniProt: P00639
• aplicaciones: cell analysis
• actividad extraña: RNase, none detected
• temp. de almacenamiento: −20°C
• Información sobre el gen: cow ... DNASE1(282217)

Descripción

Descripción general

Deoxyribonuclease I digests single- and double-stranded DNA to a mixture of mono and oligonucleotides carrying 5′phosphates and 3′OH termini. This catalytic activity is divalent ion-dependent. In the presence of Mg²⁺, DNase I hydrolyzes each strand of double-stranded DNA randomly and independently. In the presence of Mn²⁺, both strands can be cleaved.

Aplicación

Deoxyribonuclease I RNase-free solution from bovine pancreas has been used to digest DNA from various samples.

Used in molecular biology applications for removing DNA during RNA purification, for preparing DNA for nick translation, and for DNA-protein interaction analysis by footprinting methods.

Componentes

DNase I is provided in a solution of 50% (v/v) glycerol, 20 mM sodium acetate (pH 6.5), 5 mM CaCl2, and 0.1 mM PMSF.

Definición de unidad

One unit will cause a ΔA₂₆₀ of 0.001 per min per mL reaction mixture using calf thymus DNA as substrate.

Otras notas

RNA treated with this DNase I should not be used to generate a cDNA library or used for RT-PCR. For these sensitive applications, it is recommended to use DNase I, Amplification Grade , Catalog Number AMP-D1.

Información de seguridad

• Pictogramas: GHS08
• Palabra de señalización: Danger
• Frases de peligro: H334
• Consejos de prudencia: P261 - P284 - P501
• Clasificaciones de peligro: Resp. Sens. 1
• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)