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D2886

Sigma-Aldrich

DNA Ligase from T4-infected Escherichia coli

buffered aqueous glycerol solution

Sinónimos:

Polydeoxyribonucleotide Synthase, Polynucleotide Ligase, T4 DNA Ligase

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About This Item

Número de CAS:
9015-85-4
Comisión internacional de enzimas:
6.5.1.1 (BRENDA, IUBMB)
Número CE:
232-770-0
Número MDL:
MFCD00163508
Código UNSPSC:
12352204
NACRES:
NA.53

grado

for molecular biology

Nivel de calidad

200

Formulario

buffered aqueous glycerol solution

actividad específica

4,000 U/mL

mol peso

68 kDa

Nº de acceso UniProt

P00970

temp. de almacenamiento

−20°C

Información sobre el gen

bacteriophage T4 ... 30(1258680)

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Categorías relacionadas

Aplicación

Suitable for:
  • Ligation of blunt ended or cohesive DNA fragments
  • Ligation of cloning vector and restriction insert fragments
  • Seal nicks in double stranded DNA and RNA or DNA/RNA hybrids
  • Couple RNA single strands by bridging oligonucleotide adapters

Acciones bioquímicas o fisiológicas

T4 DNA Ligase forms an energy dependent phosphodiester linkage between the termini of adjacent polynucleotides of duplex DNA. The ligation reaction requires ATP as a cofactor. Ligation of blunt-ended fragments requires higher enzyme concentration and can be facilitated by using PEG in the reaction mixture. The enzyme requires a 3′ hydroxyl and 5′ phosphate for ligation. Self-ligation of vector DNA can be prevented by dephosphorylation with alkaline phosphatase. T4 ligase plays an active role in repair of DNA and RNA nicks.

Componentes

T4 DNA Ligase is supplied in a solution containing 20 mM Tris-HCl (pH 7.5), 50 mM KCl, 1 mM DTT, and 50% (v/v) glycerol.

Definición de unidad

One Weiss unit is defined as the amount of enzyme required to catalyze the exchange of 1 nmole of P32 from pyrophosphate into ATP as Norit-absorbable material in 20 minutes at 37°C.

Otras notas

T4 DNA Ligase is inactivated by heating at 65 °C for 10 minutes.

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Referencia del producto
Descripción
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P4978

Phosphatase, Alkaline from calf intestine, buffered aqueous glycerol solution

P4390

Polynucleotide Kinase from T4-infected Escherichia coli, 10 units/μL, buffered aqueous glycerol solution

G5663

Glutathione S-Transferase from E. coli, recombinant, expressed in E. coli, buffered aqueous solution

D7291

Deoxyribonuclease I RNase-free solution from bovine pancreas

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Ribonucleasa A from bovine pancreas, for molecular biology, ≥70 Kunitz units/mg protein, lyophilized

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Ribonucleasa A from bovine pancreas, (Solution of 50% glycerol, 10mM Tris-HCL pH 8.0)

P6911

Proteasa from Streptomyces griseus, BioReagent, DNase, RNase, and nickase, none detected (No RNase.)

LIG2

QuickLink™ DNA Ligation Kit, joins blunt-end and sticky-end DNA fragments

LIG1

DNA Ligation Kit

Pictogramas

Health hazard
GHS08

Palabra de señalización

Danger

Frases de peligro

H334

Consejos de prudencia

P261 - P284 - P501

Clasificaciones de peligro

Resp. Sens. 1

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificados de análisis (COA)

Lot/Batch Number
SLCL6890
SLCF8878
SLBT5956
SLBS2438V
SLBR7683V

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P Upcroft et al.
Gene, 51(1), 69-75 (1987-01-01)
We describe a system to generate cDNA or genomic libraries from DNA segments that have blunt termini. Background and rearrangement levels are low, but efficiencies are high and the procedural times very short. T4 ligase in the presence of polyethylene
Tiansheng Wang et al.
Bioorganic & medicinal chemistry letters, 22(11), 3699-3703 (2012-05-09)
A series of 4-amino-pyrido[2,3-d]pyrimidin-5(8H)-ones were designed and synthesized as a novel class of inhibitors of NAD(+)-dependent DNA ligase that possess potency against Gram-positive bacteria.
Tara Sugrue et al.
Stem cells (Dayton, Ohio), 31(1), 137-145 (2012-09-11)
The regeneration of the hematopoietic system following total body irradiation is supported by host-derived mesenchymal stromal cells (MSCs) within the bone marrow. The mechanisms used by MSCs to survive radiation doses that are lethal to the hematopoietic system are poorly
Ayako Nishizawa-Yokoi et al.
The New phytologist, 196(4), 1048-1059 (2012-10-12)
Evidence for the involvement of the nonhomologous end joining (NHEJ) pathway in Agrobacterium-mediated transferred DNA (T-DNA) integration into the genome of the model plant Arabidopsis remains inconclusive. Having established a rapid and highly efficient Agrobacterium-mediated transformation system in rice (Oryza
Athena Kantartzis et al.
Cell reports, 2(2), 216-222 (2012-09-04)
Trinucleotide repeat (TNR) expansions are the underlying cause of more than 40 neurodegenerative and neuromuscular diseases, including myotonic dystrophy and Huntington's disease. Although genetic evidence points to errors in DNA replication and/or repair as the cause of these diseases, clear
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Protocolos

T4 DNA Ligase

T4 DNA Ligase catalyzes the formation of a phosphodiester bond between the terminal 5′ phosphate and a 3′ hydroxyl groups of duplex DNA or RNA. The enzyme efficiently joins blunt and cohesive ends and repairs single-stranded nicks in duplex DNA, RNA or DNA/RNA hybrids.

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