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35845

Sigma-Aldrich

DCFHDA

BioReagent, suitable for fluorescence, ≥95% (HPLC)

Sinónimos:

2′,7′-Dichlorofluorescein diacetate, Diacetyldichlorofluorescein

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About This Item

Fórmula empírica (notación de Hill):
C24H14Cl2O7
Número de CAS:
2044-85-1
Peso molecular:
485.27
Beilstein:
68488
Número MDL:
MFCD00037501
Código UNSPSC:
12352108
ID de la sustancia en PubChem:
57649438
NACRES:
NA.32

Línea del producto

BioReagent

Nivel de calidad

100

Análisis

≥95% (HPLC)

mp

232-234 °C (lit.)

solubilidad

DMSO: soluble

fluorescencia

λex 504 nm; λem 524 nm in 0.1 M Tris pH 8.0 (esterase)

idoneidad

suitable for fluorescence

temp. de almacenamiento

2-8°C

cadena SMILES

CC(=O)Oc1cc2Oc3cc(OC(C)=O)c(Cl)cc3C4(OC(=O)c5ccccc45)c2cc1Cl

InChI

1S/C24H14Cl2O7/c1-11(27)30-21-9-19-15(7-17(21)25)24(14-6-4-3-5-13(14)23(29)33-24)16-8-18(26)22(31-12(2)28)10-20(16)32-19/h3-10H,1-2H3

Clave InChI

VQVUBYASAICPFU-UHFFFAOYSA-N

Descripción general

2′,7′-Dichlorofluorescein diacetate, also known as DCFHDA or Dcfh-DA, is a sensitive cell-permeable redox probe. The lipophilic non-fluorescent Dcfh-DA readily crosses the cell membrane through passive diffusion followed by deacetylation. The deacylated product is an oxidant sensitive 2′,7′-dichlorofluorescein (DCHF). DCHF is oxidized later to form highly fluorescent DCF, which is measured.

Aplicación

2′,7′-Dichlorofluorescein diacetate is predominantly used to detect reactive oxygen (ROS) intermediates and quantify the overall oxidative stress in cells.

Dcfh-DA is used in the quantitative assay to measure oxidative stress in nanoparticle-treated cells.

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

dust mask type N95 (US), Eyeshields, Gloves

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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