69182

DNase I, RNase free

For applications in which maintenance of RNA integrity is critical

• Sinónimos: RNase-free DNase I
• Código UNSPSC: 12352202
• NACRES: NA.85

Propiedades

• formulario: liquid
• fabricante / nombre comercial: Novagen^®
• condiciones de almacenamiento: OK to freeze; avoid repeated freeze/thaw cycles
• técnicas: DNA extraction: suitable
• idoneidad: suitable for nucleic acid purification
• aplicaciones: diagnostic assay manufacturing
• Condiciones de envío: wet ice
• temp. de almacenamiento: −20°C

Descripción

Descripción general

RNase-free DNase I digests either single- or double-stranded DNA, producing a mixture of mono- and oligonucleotides. Purified to be free of RNase, this preparation is qualified for applications in which maintenance of the integrity of RNA is critical. The enzyme selectively degrades DNA in the presence of RNA and can be used to remove DNA template following in vitro transcription reactions. This enzyme is also useful in other applications such as DNase footprinting and nick translation.

Advertencia

Toxicity: Standard Handling (A)

Definición de unidad

One unit will degrade 1 μg DNA in 10 minutes at 37°C. The reaction mixture (50 µl) contains 80 mM HEPES pH 7.5, 10 mM NaCl, 5 mM MgCl₂, 10 mM DTT, 1 µg plasmid DNA, and enzyme.

Información legal

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable